Chordin-Like 1对高糖条件下骨髓干细胞增殖、外泌体排泄和心肌损伤的调控

Hong Zhang, Liang Tao, Xinrui Zhang, Hongyan Zhang
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摘要

本研究评估了CHRDL1(Chordin-like 1)i在高糖条件下骨髓基质细胞(BMSC)增殖、外泌体排泄和心肌损伤中的作用。从高CHRDL1表达的大鼠建立BMSC。收集具有高CHRDL1水平的BMSC上清液中的外泌体。将H9C2细胞分为对照组、高糖组和exo-CHRDL1-BMSC组,然后分析CHRDL1水平、BMSC增殖和凋亡、Beclin-1、Atg5、Bcl-2和Bax的表达以及ROS和SOD活性。在exo-CHRDL1-BMSC组中,细胞增殖被促进,凋亡活性降低,ROS活性降低,SOD活性增加,Bcl-2上调,Bax下调。此外,exo-CHRDL1-BMSC组表现出CHRDL1分泌增加,Beclin-1和Atg5表达上调。总之,CHRDL1通过调节自噬促进了高糖下BMSC的增殖,并减少了细胞凋亡。
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Chordin-Like 1 Regulates Bone Marrow Stem Cell Proliferation and Excretion of Exosome and Myocardial Damage Under High Sugar
This study assesses the role of CHRDL1 (Chordin-like 1) i in the bone marrow stromal cells (BMSC) proliferation and excretion of exosome and myocardial damage under high sugar. BMSC from rats with high CHRDL1 expression was established. The exosome in the supernatant of BMSC with high CHRDL1 level was collected. H9C2 cells were assigned into control set, high sugar set and exo-CHRDL1-BMSC set followed by analysis of CHRDL1 level, BMSC proliferation and apoptosis, expression of Beclin-1, Atg5, Bcl-2 and Bax, and ROS and SOD activity. Cell proliferation was prompted and apoptotic activity was reduced in exo-CHRDL1-BMSC set with reduced ROS activity and increased SOD activity as well as upregulated Bcl-2 and downregulated Bax. In addition, exo-CHRDL1-BMSC set presented increased CHRDL1 secretion and upregulated Beclin-1 and Atg5 expression. In conclusion, proliferation of BMSC under high sugar is prompted and apoptosis is reduced by CHRDL1 through regulating the autophagy.
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