荆花胃康胶囊通过核因子- κ B信号通路对幽门螺杆菌诱导的炎症反应具有保护作用

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-06-01 DOI:10.1016/j.jtcm.2018.03.001
Shi Zongming , Ye Hui , Yu Jing , Cheng Hong , Li Jiang , Zhang Xuezhi
{"title":"荆花胃康胶囊通过核因子- κ B信号通路对幽门螺杆菌诱导的炎症反应具有保护作用","authors":"Shi Zongming ,&nbsp;Ye Hui ,&nbsp;Yu Jing ,&nbsp;Cheng Hong ,&nbsp;Li Jiang ,&nbsp;Zhang Xuezhi","doi":"10.1016/j.jtcm.2018.03.001","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H. pylori) <em>via</em> the nuclear factor-kappa B (NF-κB) signaling pathway in Kunming mice.</p></div><div><h3>METHODS</h3><p>We investigated the anti-inflammation potential of JWC extract <em>in vivo</em> in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of <em>in vivo</em> inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>RESULTS</h3><p>JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBα and NF-κB p65, mRNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.</p></div><div><h3>CONCLUSION</h3><p>An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury <em>via</em> inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway <em>in vivo.</em></p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 366-372"},"PeriodicalIF":2.0000,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.001","citationCount":"5","resultStr":"{\"title\":\"Jinghua Weikang capsule protects against Helicobacter pylori-induced inflammatory responses via the nuclear factor-kappa B signaling pathway\",\"authors\":\"Shi Zongming ,&nbsp;Ye Hui ,&nbsp;Yu Jing ,&nbsp;Cheng Hong ,&nbsp;Li Jiang ,&nbsp;Zhang Xuezhi\",\"doi\":\"10.1016/j.jtcm.2018.03.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H. pylori) <em>via</em> the nuclear factor-kappa B (NF-κB) signaling pathway in Kunming mice.</p></div><div><h3>METHODS</h3><p>We investigated the anti-inflammation potential of JWC extract <em>in vivo</em> in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of <em>in vivo</em> inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>RESULTS</h3><p>JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBα and NF-κB p65, mRNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.</p></div><div><h3>CONCLUSION</h3><p>An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury <em>via</em> inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway <em>in vivo.</em></p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":\"38 3\",\"pages\":\"Pages 366-372\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.001\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627217301395\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627217301395","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 5

摘要

目的探讨经花胃康胶囊(JWC)通过核因子κB (NF-κB)信号通路对昆明小鼠幽门螺杆菌(H. pylori)诱导的胃炎的抑制作用。方法在幽门螺杆菌诱导的小鼠胃炎模型中,研究JWC提取物的抗炎作用。免疫印迹法检测炎症相关分子的表达,酶联免疫吸附法检测体内炎症标志物的浓度。组织病理学检查炎性细胞浸润情况,定量逆转录聚合酶链反应检测相关基因mRNA表达水平。结果jwc对幽门螺杆菌诱导的胃炎具有剂量依赖性的保护作用,其机制为保护胃上皮细胞,抑制炎症细胞浸润。机制上,JWC降低了磷酸化i -κB α和NF-κB p65蛋白水平、NF-κB通路分子mRNA水平以及血浆肿瘤坏死因子-α和白细胞介素1 β水平。结论本研究的一个重要发现是JWC在体内通过抑制炎症反应和调节典型的NF-κB信号通路,减轻胃肠道炎症和溃疡,对胃损伤具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Jinghua Weikang capsule protects against Helicobacter pylori-induced inflammatory responses via the nuclear factor-kappa B signaling pathway

OBJECTIVE

To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H. pylori) via the nuclear factor-kappa B (NF-κB) signaling pathway in Kunming mice.

METHODS

We investigated the anti-inflammation potential of JWC extract in vivo in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.

RESULTS

JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBα and NF-κB p65, mRNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.

CONCLUSION

An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
期刊最新文献
Effect of Shenqi Yangxin decoction on high mobility group box 1 and inflammatory signal pathway in a rat model of dilated cardiomyopathy Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon Effect of Jianpi Bushen formula on aromatase-inhibitor-associated bone loss after menopause Effect of Cuzhi liquid on learning and memory dysfunction in a mouse model of Alzheimer's disease
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1