Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun
{"title":"长链非编码RNA LINC02532通过靶向miR-362-5p介导p-AKT调控胃癌细胞活性","authors":"Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun","doi":"10.1166/jbt.2023.3271","DOIUrl":null,"url":null,"abstract":"LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC,\n LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated.\n A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited\n migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p <\n 0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence\n of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Long Non-Coding RNA LINC02532 Mediates p-AKT to Regulate Gastric Cancer Cell Activities Through Targeting miR-362-5p\",\"authors\":\"Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun\",\"doi\":\"10.1166/jbt.2023.3271\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC,\\n LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated.\\n A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited\\n migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p <\\n 0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence\\n of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.\",\"PeriodicalId\":15300,\"journal\":{\"name\":\"Journal of Biomaterials and Tissue Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1166/jbt.2023.3271\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials and Tissue Engineering","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1166/jbt.2023.3271","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Long Non-Coding RNA LINC02532 Mediates p-AKT to Regulate Gastric Cancer Cell Activities Through Targeting miR-362-5p
LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC,
LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated.
A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited
migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p <
0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence
of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.