纳米胡芦巴口服给药阻断急性肺损伤小鼠模型中胃酸诱导的组织病理学改变、提高DNA含量和杯状细胞增殖

IF 2.8 4区医学 Q2 TOXICOLOGY Toxicology Mechanisms and Methods Pub Date : 2020-02-01 DOI:10.1080/15376516.2019.1669249

S. Hamad, M. El-Naggar

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引用次数: 2

摘要

摘要本研究旨在通过组织学和组织化学研究，检测纳米胡芦巴籽对胃酸灌胃引起的雄性瑞士白化小鼠急性肺损伤(ALI)的潜在保护作用。将40只动物分为对照组、盐酸处理组、低纳米胡芦巴+盐酸处理组、高纳米胡芦巴+盐酸处理组。在ALI动物模型中，纳米胡芦巴预处理能明显改善HCL灌注引起的肺组织病变和损伤，且呈剂量依赖性。它还能抑制HCl单独注入诱导的杯状细胞DNA含量的增加，阻止杯状细胞的增殖。综上所述，纳米胡芦巴预处理预先诱导ALI可抑制胃酸诱导的炎症细胞聚集，提高DNA含量，促进杯状细胞增殖，且呈剂量依赖性。我们认为纳米胡芦巴可能有助于对抗肺损伤。

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Blocking of gastric acid induced histopathological alterations, enhancing of DNA content and proliferation of goblet cells in the acute lung injury mice models by nano-fenugreek oral administration

Abstract This current study aimed at detecting the potential protective role of nano-fenugreek seed on acute lung injury (ALI) induced by instillation gastric acid in male Swiss albino mice using histological and histochemical studies. Forty animals were grouped as follows: control group, HCl-treated group, low nano-fenugreek + HCl treated group, and high nano-fenugreek + HCl treated group. Pretreatment with nano-fenugreek in animal model of ALI resulted in marked ameliorations of the lung histological lesions and injury induced by HCL instillation in a dose dependent manner. It also caused inhibition in the increase of the DNA content and prevented proliferation of goblet cells induced by HCl instillation alone. In conclusion, pretreatment with Nano-fenugreek prior induction ALI could be suppress the aggregations of inflammatory cells, enhancing of DNA content, and proliferation of goblet cells induced by gastric acid in a dose dependent manner. We suggest that Nano-fenugreek may be useful in combating lung injury.

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.