降糖消渴颗粒通过调节2型糖尿病小鼠肝脏内质网应激减轻糖代谢紊乱

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-08-01 DOI:10.1016/S0254-6272(18)30889-6
Zhang Yi , Mo Fangfang , Zhang Dongwei , Gao Sihua , Zhao Dandan , Yu Na , Mu Qianqian , Zuo Jiacheng , Ma Yue
{"title":"降糖消渴颗粒通过调节2型糖尿病小鼠肝脏内质网应激减轻糖代谢紊乱","authors":"Zhang Yi ,&nbsp;Mo Fangfang ,&nbsp;Zhang Dongwei ,&nbsp;Gao Sihua ,&nbsp;Zhao Dandan ,&nbsp;Yu Na ,&nbsp;Mu Qianqian ,&nbsp;Zuo Jiacheng ,&nbsp;Ma Yue","doi":"10.1016/S0254-6272(18)30889-6","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the effect of Jiangtang Xiaoke (JTXK) granule on endoplasmic reticulum (ER) stress in high fat diet (HFD)-induced type 2 diabetes mellitus (T2DM) KK-Ay mice.</p></div><div><h3>METHODS</h3><p>KK-Ay mice were fed with HFD to induce the T2DM model, while normal control C57BL/6J mice were given standard feed. Fasting blood glucose (FBG) in all mice was measured weekly and oral glucose tolerance tests (OGTTs) were performed at 4 and 10 weeks after start of treatment to determine glucose metabolism. Serum fasting insulin (FINS) and insulin sensitivity index (ISI) were measured to determine insulin sensitivity. mRNA expressions of eukaryotic initiation factor-2 alpha (eIF2α), glucose regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), and C/EBP homology protein (CHOP) were assessed by reverse transcription polymerase chain reaction and the protein expressions of p-eIF2α, GRP78, and CHOP were assessed by Western blotting.</p></div><div><h3>RESULTS</h3><p>JTXK granule significantly reduced FBG and free fatty acid levels and improved OGTT at the 120 min of the 10-week treatment in T2DM KK-Ay mice. FINS and HbAlc levels were reduced and insulin sensitivities were increased in KK-Ay diabetic mice, which were improved with the treatment of JTXK granule, especially at the 7 and 3.5 g/kg doses. JTXK granule at the 3.5 g/kg dose was most effective in reducing both gene and protein expressions of eIF2α, GRP78, and CHOP.</p></div><div><h3>CONCLUSION</h3><p>ER stress response is increased in T2DM KK-Ay mice. Treatment with JTXK granule attenuates glucose disorders, improves insulin sensitivity, and reduces serum FFA in T2DM KK-Ay mice. The mechanisms may be attributed to regulation of the signaling ER stress pathway <em>via</em> decreasing eIF2α phosphorylation and suppressing eIF2α-ATF4-CHOP activation.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 4","pages":"Pages 570-578"},"PeriodicalIF":2.0000,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30889-6","citationCount":"1","resultStr":"{\"title\":\"Jiangtang Xiaoke granule attenuates glucose metabolism disorder via regulating endoplasmic reticulum stress in the liver of type 2 diabetes mellitus mice\",\"authors\":\"Zhang Yi ,&nbsp;Mo Fangfang ,&nbsp;Zhang Dongwei ,&nbsp;Gao Sihua ,&nbsp;Zhao Dandan ,&nbsp;Yu Na ,&nbsp;Mu Qianqian ,&nbsp;Zuo Jiacheng ,&nbsp;Ma Yue\",\"doi\":\"10.1016/S0254-6272(18)30889-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To observe the effect of Jiangtang Xiaoke (JTXK) granule on endoplasmic reticulum (ER) stress in high fat diet (HFD)-induced type 2 diabetes mellitus (T2DM) KK-Ay mice.</p></div><div><h3>METHODS</h3><p>KK-Ay mice were fed with HFD to induce the T2DM model, while normal control C57BL/6J mice were given standard feed. Fasting blood glucose (FBG) in all mice was measured weekly and oral glucose tolerance tests (OGTTs) were performed at 4 and 10 weeks after start of treatment to determine glucose metabolism. Serum fasting insulin (FINS) and insulin sensitivity index (ISI) were measured to determine insulin sensitivity. mRNA expressions of eukaryotic initiation factor-2 alpha (eIF2α), glucose regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), and C/EBP homology protein (CHOP) were assessed by reverse transcription polymerase chain reaction and the protein expressions of p-eIF2α, GRP78, and CHOP were assessed by Western blotting.</p></div><div><h3>RESULTS</h3><p>JTXK granule significantly reduced FBG and free fatty acid levels and improved OGTT at the 120 min of the 10-week treatment in T2DM KK-Ay mice. FINS and HbAlc levels were reduced and insulin sensitivities were increased in KK-Ay diabetic mice, which were improved with the treatment of JTXK granule, especially at the 7 and 3.5 g/kg doses. JTXK granule at the 3.5 g/kg dose was most effective in reducing both gene and protein expressions of eIF2α, GRP78, and CHOP.</p></div><div><h3>CONCLUSION</h3><p>ER stress response is increased in T2DM KK-Ay mice. Treatment with JTXK granule attenuates glucose disorders, improves insulin sensitivity, and reduces serum FFA in T2DM KK-Ay mice. The mechanisms may be attributed to regulation of the signaling ER stress pathway <em>via</em> decreasing eIF2α phosphorylation and suppressing eIF2α-ATF4-CHOP activation.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":\"38 4\",\"pages\":\"Pages 570-578\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30889-6\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218308896\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218308896","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 1

摘要

目的观察降糖消渴颗粒对高脂饮食(HFD)诱导的2型糖尿病(T2DM) KK-Ay小鼠内质网应激的影响。方法skk - ay小鼠用HFD诱导T2DM模型,正常对照C57BL/6J小鼠用标准饲料。每周测量所有小鼠的空腹血糖(FBG),并在治疗开始后4周和10周进行口服葡萄糖耐量试验(ogtt)以测定葡萄糖代谢。测定血清空腹胰岛素(FINS)和胰岛素敏感性指数(ISI)以确定胰岛素敏感性。逆转录聚合酶链反应检测真核起始因子-2 α (eIF2α)、葡萄糖调节蛋白78 (GRP78)、激活转录因子4 (ATF4)和C/EBP同源蛋白(CHOP) mRNA表达,Western blotting检测p-eIF2α、GRP78和CHOP蛋白表达。结果jtxk颗粒可显著降低T2DM KK-Ay小鼠FBG和游离脂肪酸水平,改善OGTT。JTXK颗粒可降低KK-Ay糖尿病小鼠的FINS和HbAlc水平,提高胰岛素敏感性,且在7和3.5 g/kg剂量下效果明显。以3.5 g/kg剂量的JTXK颗粒降低eIF2α、GRP78、CHOP基因和蛋白表达最为有效。结论T2DM KK-Ay小鼠应激反应增强。JTXK颗粒治疗可减轻T2DM KK-Ay小鼠的血糖紊乱,改善胰岛素敏感性,降低血清FFA。其机制可能是通过降低eIF2α磷酸化和抑制eIF2α- atf4 - chop活化来调节内质网应激信号通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Jiangtang Xiaoke granule attenuates glucose metabolism disorder via regulating endoplasmic reticulum stress in the liver of type 2 diabetes mellitus mice

OBJECTIVE

To observe the effect of Jiangtang Xiaoke (JTXK) granule on endoplasmic reticulum (ER) stress in high fat diet (HFD)-induced type 2 diabetes mellitus (T2DM) KK-Ay mice.

METHODS

KK-Ay mice were fed with HFD to induce the T2DM model, while normal control C57BL/6J mice were given standard feed. Fasting blood glucose (FBG) in all mice was measured weekly and oral glucose tolerance tests (OGTTs) were performed at 4 and 10 weeks after start of treatment to determine glucose metabolism. Serum fasting insulin (FINS) and insulin sensitivity index (ISI) were measured to determine insulin sensitivity. mRNA expressions of eukaryotic initiation factor-2 alpha (eIF2α), glucose regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), and C/EBP homology protein (CHOP) were assessed by reverse transcription polymerase chain reaction and the protein expressions of p-eIF2α, GRP78, and CHOP were assessed by Western blotting.

RESULTS

JTXK granule significantly reduced FBG and free fatty acid levels and improved OGTT at the 120 min of the 10-week treatment in T2DM KK-Ay mice. FINS and HbAlc levels were reduced and insulin sensitivities were increased in KK-Ay diabetic mice, which were improved with the treatment of JTXK granule, especially at the 7 and 3.5 g/kg doses. JTXK granule at the 3.5 g/kg dose was most effective in reducing both gene and protein expressions of eIF2α, GRP78, and CHOP.

CONCLUSION

ER stress response is increased in T2DM KK-Ay mice. Treatment with JTXK granule attenuates glucose disorders, improves insulin sensitivity, and reduces serum FFA in T2DM KK-Ay mice. The mechanisms may be attributed to regulation of the signaling ER stress pathway via decreasing eIF2α phosphorylation and suppressing eIF2α-ATF4-CHOP activation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
期刊最新文献
Effect of Shenqi Yangxin decoction on high mobility group box 1 and inflammatory signal pathway in a rat model of dilated cardiomyopathy Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon Effect of Jianpi Bushen formula on aromatase-inhibitor-associated bone loss after menopause Effect of Cuzhi liquid on learning and memory dysfunction in a mouse model of Alzheimer's disease
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1