莪术灭活棘球蚴原球茎的有效性和安全性

IF 3.2 Q2 Pharmacology, Toxicology and Pharmaceutics Current clinical pharmacology Pub Date : 2020-04-01 DOI:10.2174/1574884714666190918155147
H. Mahmoudvand, Mahbobeh Pakravanan, F. Kheirandish, S. Jahanbakhsh, M. Sepahvand, M. Niazi, Abdolreza Rouientan, Mohammad Reza Aflatoonian
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引用次数: 12

摘要

背景:本研究旨在评价莪术精油的化学成分,并探讨其对棘球蚴原头节的疗效和安全性。方法从感染绵羊的肝可育性棘球蚴中采集原头节细胞,将其暴露于不同浓度的精油(75150300µl/mL)中5-30 min。然后,用曙红排斥法对原头节细胞的活力进行了研究。在下一步中,对24只雄性NMRI小鼠进行检查,通过测量生化和血液学参数来评估莪术精油的毒性。结果腹腔注射莪术精油的LD50值为1.76 mL/kg体重,最大非致死剂量为0.96 mL/kg体重。C.zadoaria精油在体外具有强大的原scolicidal活性,因此在300和150µl/ml的浓度下,5分钟和10分钟后完全消除寄生虫;而在较低剂量下观察到弱的原scolicidal活性。离体测定,没有观察到与体外相似的效果,因此,需要更多的时间才能显示出有效的原scolicidal活性。在暴露时间为7分钟和12分钟后,浓度为300和150µl/mL的莪术精油分别杀死了棘球蚴内100%的原头节。腹腔注射莪术精油2周后,差异无统计学意义(p > 0.05)在0.15、0.3、0.6剂量下的临床化学和血液学参数中观察到 mL/kg。结论体外和离体实验结果表明,莪术挥发油对棘球蚴原节具有良好的原节活性。然而,还需要更多的补充工作来通过评估临床受试者来验证这些发现。
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Efficacy and Safety Curcuma zadoaria L. to Inactivate the Hydatid Cyst Protoscoleces
Background: The present work aimed to evaluate the chemical composition of Curcuma zadoaria essential oil and to investigate its efficacy and safety against hydatid cyst protoscoleces. Methods Collected protoscoleces from liver fertile hydatid cysts of infected sheep were exposed to different concentrations of the essential oil (75, 150, 300 µl/mL) for 5-30 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied. In the next step, 24 male NMRI mice were examined to assess the toxicity of C. zadoaria essential oil by measuring the biochemical and hematological parameters. Results Based on the obtained results, the LD50 value of intraperitoneal injection of the C. zadoaria essential oil was 1.76 mL/kg of body weight and the maximum non-fatal dose was 0.96 mL/kg of body weight. C. zadoaria essential oil had a strong proto scolicidal activity in vitro so that at the 300 and 150 µl/ml entirely eliminates the parasite after 5 and 10 minutes; whereas, weak proto scolicidal activity was observed at lower doses. Ex vivo assay, no similar effect with in vitro was observed, therefore, more time is required to show a potent proto scolicidal activity. C. zadoaria essential oil at the concentrations of 300 and 150 µl/mL after an exposure time of 7 and 12 min, killed 100% of protoscoleces within the hydatid cyst, respectively. After intraperitoneal injection of the C. zadoaria essential oil for 2 weeks, no significant difference (p > 0.05) was observed in the clinical chemistry and hematologic parameters at the doses of 0.15, 0.3, 0.6 mL/kg. Conclusion The obtained results in vitro and ex vivo exhibited that C. zadoaria essential oil had a favorable proto scolicidal activity on hydatid cyst protoscoleces. However, more supplementary works are required to verify these findings by assessing clinical subjects.
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Current clinical pharmacology
Current clinical pharmacology PHARMACOLOGY & PHARMACY-
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期刊介绍: Current Clinical Pharmacology publishes frontier reviews on all the latest advances in clinical pharmacology. The journal"s aim is to publish the highest quality review articles in the field. Topics covered include: pharmacokinetics; therapeutic trials; adverse drug reactions; drug interactions; drug metabolism; pharmacoepidemiology; and drug development. The journal is essential reading for all researchers in clinical pharmacology.
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