采用双水相体系一步芯片微流控合成杂化胶囊

Sneha Daradmare, Jae Seong Kim, R. Ganguly, Chang-Soo Lee
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摘要

由于使用油和表面活性剂,传统油包水乳液系统合成的水凝胶胶囊在生物医学应用中不太受欢迎。水两相体系(ATPS)可以形成水包水乳液,被认为是一种绿色的替代方案,因此在生物医学领域的应用得到了大量的探索。在这里,我们提出了水凝胶胶囊的合成使用的设置组成的气动阀集成的ATPS微流体系统。在这种安排中,首先,气动阀促进一个水系统的液滴的产生,即含有葡聚糖溶液的海藻酸钠(SA)进入另一个含有聚乙二醇溶液的水相。目前的方法允许通过调整气动阀的压力和核心和中间相的流量来很好地控制液滴的产生。微流控装置内杂化胶囊的合成主要是利用带相反电荷的聚电解质壳聚糖与SA通过静电相互作用进行界面络合。采用沉降收集法收集界面络合的SA和壳聚糖水凝胶胶囊,保证了杂化胶囊的形状不变。通过光学显微镜观察了合成的微滴和杂交胶囊的形态特征。水凝胶胶囊对磁性颗粒具有良好的包封能力。尽管本研究主要集中在合成部分,但我们预计所提出的方法将使细胞被封装在杂交胶囊内,并增强细胞在水凝胶胶囊表面的粘附,因此这些水凝胶胶囊可以在生物医学工程中找到强有力的应用。
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One-step on-chip microfluidic synthesis of the hybrid capsules using aqueous two-phase system
Hydrogel capsules synthesized by conventional water-in-oil emulsion systems are the less preferred choice for biomedical applications due to the use of oils and surfactants. An aqueous two-phase system (ATPS), which allows the formation of water-in-water emulsion, is considered a green alternative and therefore has been explored a lot for its application in the biomedical field. Herein, we present the synthesis of hydrogel capsules using a set-up consisting of a pneumatic valve integrated with the ATPS microfluidic system. In this arrangement, at first, a pneumatic valve facilitates the generation of the droplets of one aqueous system i.e. sodium alginate (SA) containing dextran solution into another aqueous phase comprising polyethylene glycol solution. The present approach allows good control over droplet generation by tuning the pressure of the pneumatic valve and the flow rates of the core and middle phases. The synthesis of hybrid capsules within the microfluidic device is carried out mainly by using the interfacial complexation of oppositely charged polyelectrolytes, chitosan with SA via electrostatic interactions. The interfacial complexed SA and chitosan hydrogel capsules were collected via the settling collection method, which ensures the retaining of the shape of the hybrid capsules. The morphological properties of as-synthesized droplets and hybrid capsules were examined via optical microscopy. The hydrogel capsules show good encapsulation capability for the magnetic particles. Even though this study mainly focuses on the synthesis part, we anticipate that the proposed approach will enable the encapsulation of cells within the hybrid capsules as well as enhance the cell adhesion on the surface of the hydrogel capsules hence, these hydrogel capsules can find the potent application in the biomedical engineering.
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