Francisco Javier Preciado-Gutiérrez, David Masuoka-Ito, José Luis Barrera-Bernal, Bryan Ivan Martín del Campo-Téllez, Vicente Esparza-Villalpando, Ricardo-Ernesto Ramírez-Orozco
{"title":"墨西哥肉类工业胎儿牛血清细胞培养的特性","authors":"Francisco Javier Preciado-Gutiérrez, David Masuoka-Ito, José Luis Barrera-Bernal, Bryan Ivan Martín del Campo-Téllez, Vicente Esparza-Villalpando, Ricardo-Ernesto Ramírez-Orozco","doi":"10.22319/rmcp.v14i2.6277","DOIUrl":null,"url":null,"abstract":"The present work aims to characterize a fetal bovine serum (FBS) obtained from the meat industry for use in cell culture. FBS is the most widely used supplement for cell culture since its complex composition provides the necessary nutrients for the growth of most cells. It is a by-product of the meat industry, and its availability and production depend mainly on two uncontrollable external factors, climatic conditions and changes in beef consumption. According to the strict quality features of the International Serum Industry Association (ISIA), tests for total proteins, osmolarity, presence or absence of pathogenic biological agents, pH, DNA concentration, biological contaminants, negative results, and cell viability were performed. The characterization of the serum in the DNA and total protein concentration tests showed significant differences. Additionally, osmolarity and pH did not present significant differences between groups. Regarding the viability test, no complication for cell growth was observed despite the differences found in the characterization. The results showed that the serum obtained from the meat industry could maintain cell cultures and allow cell proliferation compared to commercial serum. Furthermore, if FBS is not available, some kinds of plasma can be used as a surrogate to maintain cell cultures.","PeriodicalId":21443,"journal":{"name":"Revista Mexicana de Ciencias Pecuarias","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Caracterización del suero bovino fetal proveniente de la industria cárnica mexicana en el cultivo celular\",\"authors\":\"Francisco Javier Preciado-Gutiérrez, David Masuoka-Ito, José Luis Barrera-Bernal, Bryan Ivan Martín del Campo-Téllez, Vicente Esparza-Villalpando, Ricardo-Ernesto Ramírez-Orozco\",\"doi\":\"10.22319/rmcp.v14i2.6277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present work aims to characterize a fetal bovine serum (FBS) obtained from the meat industry for use in cell culture. FBS is the most widely used supplement for cell culture since its complex composition provides the necessary nutrients for the growth of most cells. It is a by-product of the meat industry, and its availability and production depend mainly on two uncontrollable external factors, climatic conditions and changes in beef consumption. According to the strict quality features of the International Serum Industry Association (ISIA), tests for total proteins, osmolarity, presence or absence of pathogenic biological agents, pH, DNA concentration, biological contaminants, negative results, and cell viability were performed. The characterization of the serum in the DNA and total protein concentration tests showed significant differences. Additionally, osmolarity and pH did not present significant differences between groups. Regarding the viability test, no complication for cell growth was observed despite the differences found in the characterization. The results showed that the serum obtained from the meat industry could maintain cell cultures and allow cell proliferation compared to commercial serum. Furthermore, if FBS is not available, some kinds of plasma can be used as a surrogate to maintain cell cultures.\",\"PeriodicalId\":21443,\"journal\":{\"name\":\"Revista Mexicana de Ciencias Pecuarias\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Mexicana de Ciencias Pecuarias\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.22319/rmcp.v14i2.6277\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Mexicana de Ciencias Pecuarias","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22319/rmcp.v14i2.6277","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Caracterización del suero bovino fetal proveniente de la industria cárnica mexicana en el cultivo celular
The present work aims to characterize a fetal bovine serum (FBS) obtained from the meat industry for use in cell culture. FBS is the most widely used supplement for cell culture since its complex composition provides the necessary nutrients for the growth of most cells. It is a by-product of the meat industry, and its availability and production depend mainly on two uncontrollable external factors, climatic conditions and changes in beef consumption. According to the strict quality features of the International Serum Industry Association (ISIA), tests for total proteins, osmolarity, presence or absence of pathogenic biological agents, pH, DNA concentration, biological contaminants, negative results, and cell viability were performed. The characterization of the serum in the DNA and total protein concentration tests showed significant differences. Additionally, osmolarity and pH did not present significant differences between groups. Regarding the viability test, no complication for cell growth was observed despite the differences found in the characterization. The results showed that the serum obtained from the meat industry could maintain cell cultures and allow cell proliferation compared to commercial serum. Furthermore, if FBS is not available, some kinds of plasma can be used as a surrogate to maintain cell cultures.
期刊介绍:
The MEXICAN MAGAZINE OF SCIENCES PECUARIAS is an organ of scientific and technical diffusion of the livestock sector. Its periodicity is quarterly and arbitrated by pairs in the double-blind mode. Its objective is to make known the results of the research carried out by any scientific institution, in Mexico and in any part of the world, related to the livestock sciences, particularly those that refer to the different disciplines of Veterinary Medicine and Animal Science. The Journal is bilingual, publishes the complete articles in Spanish or English and is included in various indexing services and international dissemination platforms, such as the Index of Mexican Journals of Scientific and Technological Research of the National Council of Science and Technology (CONACYT); In the EBSCO Host database; In the Network of Scientific Journals of Latin America and the Caribbean, Spain and Portugal (RedALyC); In the Ibero-American Network of Scientific Journals of Free Access Veterinary Medicine. Indexed in the ISI Journal Citation Report Science Edition; And Elsevier''s SCOPUS and EMBASE indices.