姜与特朗花组合茶的抗氧化活性

IF 0.1 Q4 MEDICINE, GENERAL & INTERNAL Majalah Kedokteran Bandung-MKB-Bandung Medical Journal Pub Date : 2022-09-01 DOI:10.15395/mkb.v54n3.2729
W. Widowati, T. Wargasetia, Teddy Marcus Zakaria, Meganita Marthania, Michael Sebastian Gunadi, Nathanael Halim, Nindia Salsabila Mia Dewi, Sherly Santiadi
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引用次数: 1

摘要

茶与咖啡和可可一起被誉为全球最受欢迎的饮料之一。茶已被用作保健品或药物来预防和治疗各种疾病。凉茶已经成为人们最受欢迎的饮料之一,经常被人们用于健康目的。本文报道了一种由干姜和特朗花(Clitoria ternatea)制成的凉茶组合的抗氧化活性。该实验于2021年11月至2021年12月底在印度尼西亚西爪哇万隆Aretha Medika Utama生物分子和生物医学研究中心的普通实验室进行。JaTe茶是通过将2克干姜和5个芽特朗花在200毫升中煮沸5分钟制成的。对姜干茶、特朗干茶和翡翠茶的抗氧化活性进行了评价。总酚类和类黄酮含量通过Folin-Ciocalte使用没食子酸当量(GAE)进行评估,而类黄酮含量使用槲皮素当量(QE)和氯化铝比色法进行评估。用2,2-二苯基-1-苦基肼(DPPH)和2,2'-叠氮基-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)测定抗氧化活性,用铁还原抗氧化能力(FRAP)测定过氧化氢(H2O2)清除活性。这项研究表明,姜茶、特朗茶和JaTe茶配方具有抗氧化活性,因为其IC50值较低。姜茶中的酚类和黄酮类含量最高,GAE/100%浓度为23.62μg,其次是特朗茶,QE/100%浓度为7.22μg。JaTe茶具有最强的ABTS清除活性,IC50为1.01%,FRAP活性为3.59%。同时,姜茶对DPPH的清除活性最强,IC50值为7.76%,对H2O2的清除活性为13.23%。总体而言,JaTe凉茶配方具有很强的抗氧化活性。
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Antioxidant Activities of Ginger (Zingiber officinale Roscoe) and Telang Flower (Clitoria ternatea L.) Combination Tea
Tea is known as one of the favorite beverages globally, together with coffee and cocoa. Tea has been used as a health product or medicinal drug to prevent and treat various diseases. Herbal tea has become one of the most sought-after beverages often consumed by the people for health purposes. This paper reports the antioxidant activities of a herbal tea combination from dried ginger and telang flower (Clitoria ternatea), which is referred to as JaTe in Indonesian, as the abbreviation of Jahe (Ginger, Zingiber officinale) and Telang flower (Clitoria ternatea). The experiment was conducted at the general laboratory of the Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia, during the period of November 2021 to the end of December 2021. The JaTe tea was made by combining 2 g dried ginger and 5 buds telang flower that were boiled in 200 mL for 5 minutes. The dried ginger tea, dried telang tea, and JaTe tea were evaluated for the antioxidant activities. The total phenolic and flavonoid content was evaluated by Folin-Ciocalte using the Gallic Acid Equivalent (GAE), while the flavonoid content was evaluated using the Quercetin Equivalent (QE) and the aluminum chloride colorimetric assay. The antioxidant activity was measured using 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2'-Azino-Bis-3-Ethylbenzothiazoline-6-sulfonic acid (ABTS) while the hydrogen peroxide (H2O2) scavenging activities was measured using ferric reducing antioxidant power (FRAP) assay. This study showed that ginger tea, telang tea, and JaTe tea formulations have antioxidant activities due to its low IC50 values. The highest phenolic content and flavonoid content was counx in ginger tea with 23.62 μg GAE/100% concentration, followed by telang tea with 7.22 μg QE/100% concentration. JaTe tea has the strongest ABTS scavenging activity with IC50= 1.01% and FRAP activity with IC50=3.59 %. Meanwhile, the ginger tea has the strongest DPPH scavenging activity with an IC50 value of 7.76 % and an H2O2 scavenging activity with IC50=13.23 %. Overall, JaTe herbal tea formulation has a strong antioxidant activity.
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