Siran Wang, Shiwei Hu, Zhihao Dong, Junfeng Li, Jie Zhao, Mudasir Nazar, Niaz Ali Kaka, Tao Shao
{"title":"燕麦和意大利黑麦草附生菌群对玉米青贮发酵产物和细菌群落结构的贡献","authors":"Siran Wang, Shiwei Hu, Zhihao Dong, Junfeng Li, Jie Zhao, Mudasir Nazar, Niaz Ali Kaka, Tao Shao","doi":"10.1186/s40538-023-00439-y","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.</p><h3>Results</h3><p>MZMZ had higher (<i>P</i> < 0.05) lactic acid contents, and lower (<i>P</i> < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of <i>Lactobacillus</i> in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (<i>P</i> < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (<i>P</i> < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.</p><h3>Conclusions</h3><p>The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, <i>Hafnia-Obesumbacterium</i>, hetero-fermentative and acid-resistant <i>Lactobacillus</i> took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.</p><h3>Graphical Abstract</h3>\n <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\n </div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"10 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-023-00439-y","citationCount":"0","resultStr":"{\"title\":\"The contribution of epiphytic microbiota in oat and Italian ryegrass to silage fermentation products and bacterial community structure of whole-crop maize\",\"authors\":\"Siran Wang, Shiwei Hu, Zhihao Dong, Junfeng Li, Jie Zhao, Mudasir Nazar, Niaz Ali Kaka, Tao Shao\",\"doi\":\"10.1186/s40538-023-00439-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.</p><h3>Results</h3><p>MZMZ had higher (<i>P</i> < 0.05) lactic acid contents, and lower (<i>P</i> < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of <i>Lactobacillus</i> in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (<i>P</i> < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (<i>P</i> < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.</p><h3>Conclusions</h3><p>The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, <i>Hafnia-Obesumbacterium</i>, hetero-fermentative and acid-resistant <i>Lactobacillus</i> took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.</p><h3>Graphical Abstract</h3>\\n <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\\n </div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2023-07-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-023-00439-y\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-023-00439-y\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-023-00439-y","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
The contribution of epiphytic microbiota in oat and Italian ryegrass to silage fermentation products and bacterial community structure of whole-crop maize
Background
The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.
Results
MZMZ had higher (P < 0.05) lactic acid contents, and lower (P < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of Lactobacillus in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (P < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (P < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.
Conclusions
The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, Hafnia-Obesumbacterium, hetero-fermentative and acid-resistant Lactobacillus took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
