不同Led光照条件下培养的钝顶螺旋藻的生长、繁殖率和抗氧化活性

IF 0.5 4区 地球科学 Q3 Earth and Planetary Sciences Journal of Marine Research Pub Date : 2022-11-12 DOI:10.14710/jmr.v11i4.35310
Budi Aryono, Muhammad Zainuddin, Risha Fillah Fithria
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Perlakuan terbaik adalah pemberian pencahayaan LED merah yaitu Spirulina platensis memiliki pertumbuhan mutlak sebesar 1,602 d x 104 sel/ml, laju pertumbuhan 0,108 hari-1, jumlah generasi 2,028, waktu generasi 6,416 hari, yield 9,649 gr/gr, dan produktifitas 24,740 gr/L.hari. Ekstrak spirulina mengandung klorofil a sebesar 10,153 mg/L, klorofil b sebesar 4,744 mg/L, total klorofil 16,053 mg/L, karotenoid 5,893 mg/L. selain itu juga memiliki kandungan pigmen fikobiliprotein yaitu fikosianin 0,055 mg/L, alofikosianin 0,037 mg/L dan fikoeritrin 0,218 mg/L. eksrak spirulina dari hasil kultur dengan pencahayaan LED merah memiliki aktivitas antioksidan dengan nilai IC50 sebesar 105,4 ppm. Aktivitas antioksidan dari ekstrak sepirulina di karenakan adanya kandungan senyawa fitokimia dan pigmen. Antioxidants are needed by the body to protect the health of our skin. One source of antioxidants is the microalgae spirulina sp. 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引用次数: 0

摘要

身体需要抗氧化剂来保护我们的皮肤健康。螺旋藻是抗氧化剂的来源之一。微藻。使用螺旋藻的问题是,它需要正确的培养技术才能产生高抗氧化活性。该研究的目的是优化螺旋藻培养中的光照(浅色),以实现最佳的生长、色素率和最高的抗氧化活性。这项研究使用了5种处理的实验室实验方法,蓝色、绿色、红色、白色和荧光LED都具有可信度。培养物中不同颜色的光对生长、色素生成率和抗氧化活性有着真正的影响。最佳实践是发出红色LED光,即钝顶螺旋藻的绝对生长量为1602d×104个细胞/ml,生长速率为0.108天-1,代数为2028,代数2028,世代时间为6416天,产量为9649 gr/gr,生产率为24740 gr/L.3 mg/L,类胡萝卜素5893 mg/L。此外,它还含有一种五倍子蛋白色素,即0.055mg/L、0.037mg/L和0.218mg/L。来自具有红色LED照明的培养结果的螺旋藻提取物具有IC50值为105.4ppm的抗氧化活性。由于存在植物化学化合物和色素,海泡石提取物具有抗氧化活性。身体需要抗氧化剂来保护皮肤健康。抗氧化剂的一个来源是微藻螺旋藻。使用螺旋藻的问题是需要适当的培养技术来产生高抗氧化活性。本研究的目的是优化螺旋藻培养中的光照(浅色),以获得最佳的生长、最高的色素含量和抗氧化活性。这项研究采用了一种实验室实验方法,共有5种处理方法,分别用蓝色、绿色、红色、白色LED和荧光灯照明。培养基中不同光照颜色的处理对生长、色素水平和抗氧化活性有显著影响。最佳处理是提供红色LED照明,螺旋藻的绝对生长量为1602d×104个细胞/ml,生长速率为0108天-1,世代数为2028,世代时间为6416天,产量为9649克/克,生产力为24740克/升,类胡萝卜素5893 mg/L。此外,它还含有藻胆蛋白色素、藻蓝蛋白0.055mg/L、别藻蓝蛋白0.037mg/L和藻红蛋白0.218mg/L。用红色LED照明培养的螺旋藻提取物具有抗氧化活性,IC50值为105.4ppm。海泡石提取物的抗氧化活性是由于存在植物化学化合物和色素。
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Pertumbuhan, Kadar Pigmen dan Aktivitas Antioksidan Spirulina platensis pada Kultur dengan Perbedaan Warna Pencahayaan Leds
Antioksidan diperlukan tubuh untuk melindungi kesehatan kulit kita. Salah satu sumber antioksidan adalah mikroalga Spirulina sp. Permasalahan pemanfaatan spirulina adalah diperlukan teknologi kultur yang tepat untuk menghasilkan aktivitas antioksidan tinggi. Tujuan penelitian adalah melakukan optimasi pencahayaan (warna cahaya) dalam kultur spirulina untuk mendapatkan pertumbuhan terbaik, kadar pigmen dan aktivitas antioksidan tertinggi. Penelitian menggunakan metode eksperimen laboratoris dengan 5 perlakuan yaitu pemberian pencahaaan dengan LED warna biru, hijau, merah, putih dan lampu TL (fluoresens). Perlakuan perbedaan warna pencahayaan dalam kultur berpengaruh nyata terhadap pertumbuhan, kadar pigmen dan aktivitas antioksidan. Perlakuan terbaik adalah pemberian pencahayaan LED merah yaitu Spirulina platensis memiliki pertumbuhan mutlak sebesar 1,602 d x 104 sel/ml, laju pertumbuhan 0,108 hari-1, jumlah generasi 2,028, waktu generasi 6,416 hari, yield 9,649 gr/gr, dan produktifitas 24,740 gr/L.hari. Ekstrak spirulina mengandung klorofil a sebesar 10,153 mg/L, klorofil b sebesar 4,744 mg/L, total klorofil 16,053 mg/L, karotenoid 5,893 mg/L. selain itu juga memiliki kandungan pigmen fikobiliprotein yaitu fikosianin 0,055 mg/L, alofikosianin 0,037 mg/L dan fikoeritrin 0,218 mg/L. eksrak spirulina dari hasil kultur dengan pencahayaan LED merah memiliki aktivitas antioksidan dengan nilai IC50 sebesar 105,4 ppm. Aktivitas antioksidan dari ekstrak sepirulina di karenakan adanya kandungan senyawa fitokimia dan pigmen. Antioxidants are needed by the body to protect the health of our skin. One source of antioxidants is the microalgae spirulina sp. The problem of using spirulina is that appropriate culture technology is needed to produce high antioxidant activity. The aim of the research was to optimize lighting (light color) in spirulina culture to get the best growth, highest pigment content and antioxidant activity. The study used a laboratory experimental method with 5 treatments, lighting with blue, green, red, white LEDs and fluorescent lights. The treatment of different lighting colors in culture had a significant effect on growth, pigment levels and antioxidant activity. The best treatment was the provision of red LED lighting, Spirulina platensis which had absolute growth of 1.602 d x 104 cells/ml, growth rate of 0.108 day-1 , number of generations 2,028, generation time of 6.416 days, yield of 9.649 gr/gr, and productivity of 24.740 gr/L.day. Spirulina extract contains chlorophyll a of 10,153 mg/L, chlorophyll b of 4,744 mg/L, total chlorophyll 16,053 mg/L, carotenoids 5,893 mg/L. In addition, it also contains phycobiliprotein pigments, phycocyanin 0.055 mg/L, allophycocyanin 0.037 mg/L and phycoerythrin 0.218 mg/L. Spirulina extract from culture results with red LED lighting has antioxidant activity with an IC50 value of 105.4 ppm. The antioxidant activity of the sepirulina extract is due to the presence of phytochemical compounds and pigments.
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Journal of Marine Research
Journal of Marine Research 地学-海洋学
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3 months
期刊介绍: The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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