Jialu Wang, Kudelaiti Maidinayi, Wenjing Tang, Li-ping Lu, R. Xu, Y. Wan
{"title":"表没食子儿茶素没食子酸酯对高脂饮食大鼠白色脂肪组织血管生成的影响及其机制","authors":"Jialu Wang, Kudelaiti Maidinayi, Wenjing Tang, Li-ping Lu, R. Xu, Y. Wan","doi":"10.3760/CMA.J.ISSN.1674-635X.2018.06.008","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effects and mechanism of (-)-epigallocatechin-3-gallate (EGCG) on white adipose tissue angiogenesis in high fat diet rats. \n \n \nMethods \nTwenty-four male weaning SD rats were randomly divided into normal control group, high fat diet group and EGCG intervention group, 8 rats in each group. Normal control group were fed with normal diet, high-fat diet group were fed with high-fat diet, EGCG intervention group were fed with high-fat diet along with intragastric administration of 200 mg/(kg·d) EGCG. After 8 weeks, the rats were sacrificed. The adipocyte size and vascular density of the abdominal adipose tissue in rats in each group were observed under the microscope.The serum vascular endothelial growth factor (VEGF) concentration was detected by Elisa Kit. RT-PCR was used to detect the expression of VEGF, nuclear factor E2 (Nrf2), heme oxygenase-1 (HO-1), catalase (CAT), SOD, GPx, interleukin-6(IL-6) and monocyte chemoattractant protein-1 (MCP-1) mRNA. \n \n \nResults \nThe adipocyte size, number of vascular/each adipocyte, serum VEGF concentration and VEGF mRNA expression in adipose tissue of high fat diet group were significantly higher than those of normal control group (all P<0.05). EGCG can significantly reduce the above indicators of high fat diet group (all P<0.05). The expression of Nrf2, HO-1, SOD, GPx and CAT mRNA in adipose tissue of EGCG group was significantly higher than those in high fat diet group and normal control group (all P<0.05). The expression of MCP-1 and IL-6 mRNA in adipose tissue of EGCG group was significantly lower than that in high fat diet group (all P<0.05). \n \n \nConclusion \nEGCG can decrease the production of serum VEGF, vascular density and the expression of VEGF mRNA in white adipose tissue of high fat diet rats, and inhibit the angiogenesis in white adipose tissue possibly due to its up-regulation of Nrf2/HO-1 pathway to increase the expression of antioxidant enzymes (SOD, CAT, GPx), reduce ROS production and decrease the inflammatory response. \n \n \nKey words: \n(-)-Epigallocatechin-3-gallate; High fat diet; White adipose tissue; Angiogenesis; Vascular endothelial growth factor","PeriodicalId":9877,"journal":{"name":"中华临床营养杂志","volume":"26 1","pages":"366-372"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effects and mechanism of epigallocatechin gallate on white adipose tissue angiogenesis in high fat diet rats\",\"authors\":\"Jialu Wang, Kudelaiti Maidinayi, Wenjing Tang, Li-ping Lu, R. Xu, Y. Wan\",\"doi\":\"10.3760/CMA.J.ISSN.1674-635X.2018.06.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effects and mechanism of (-)-epigallocatechin-3-gallate (EGCG) on white adipose tissue angiogenesis in high fat diet rats. \\n \\n \\nMethods \\nTwenty-four male weaning SD rats were randomly divided into normal control group, high fat diet group and EGCG intervention group, 8 rats in each group. Normal control group were fed with normal diet, high-fat diet group were fed with high-fat diet, EGCG intervention group were fed with high-fat diet along with intragastric administration of 200 mg/(kg·d) EGCG. After 8 weeks, the rats were sacrificed. The adipocyte size and vascular density of the abdominal adipose tissue in rats in each group were observed under the microscope.The serum vascular endothelial growth factor (VEGF) concentration was detected by Elisa Kit. RT-PCR was used to detect the expression of VEGF, nuclear factor E2 (Nrf2), heme oxygenase-1 (HO-1), catalase (CAT), SOD, GPx, interleukin-6(IL-6) and monocyte chemoattractant protein-1 (MCP-1) mRNA. \\n \\n \\nResults \\nThe adipocyte size, number of vascular/each adipocyte, serum VEGF concentration and VEGF mRNA expression in adipose tissue of high fat diet group were significantly higher than those of normal control group (all P<0.05). EGCG can significantly reduce the above indicators of high fat diet group (all P<0.05). The expression of Nrf2, HO-1, SOD, GPx and CAT mRNA in adipose tissue of EGCG group was significantly higher than those in high fat diet group and normal control group (all P<0.05). The expression of MCP-1 and IL-6 mRNA in adipose tissue of EGCG group was significantly lower than that in high fat diet group (all P<0.05). \\n \\n \\nConclusion \\nEGCG can decrease the production of serum VEGF, vascular density and the expression of VEGF mRNA in white adipose tissue of high fat diet rats, and inhibit the angiogenesis in white adipose tissue possibly due to its up-regulation of Nrf2/HO-1 pathway to increase the expression of antioxidant enzymes (SOD, CAT, GPx), reduce ROS production and decrease the inflammatory response. \\n \\n \\nKey words: \\n(-)-Epigallocatechin-3-gallate; High fat diet; White adipose tissue; Angiogenesis; Vascular endothelial growth factor\",\"PeriodicalId\":9877,\"journal\":{\"name\":\"中华临床营养杂志\",\"volume\":\"26 1\",\"pages\":\"366-372\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华临床营养杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1674-635X.2018.06.008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Nursing\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华临床营养杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1674-635X.2018.06.008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Nursing","Score":null,"Total":0}
Effects and mechanism of epigallocatechin gallate on white adipose tissue angiogenesis in high fat diet rats
Objective
To investigate the effects and mechanism of (-)-epigallocatechin-3-gallate (EGCG) on white adipose tissue angiogenesis in high fat diet rats.
Methods
Twenty-four male weaning SD rats were randomly divided into normal control group, high fat diet group and EGCG intervention group, 8 rats in each group. Normal control group were fed with normal diet, high-fat diet group were fed with high-fat diet, EGCG intervention group were fed with high-fat diet along with intragastric administration of 200 mg/(kg·d) EGCG. After 8 weeks, the rats were sacrificed. The adipocyte size and vascular density of the abdominal adipose tissue in rats in each group were observed under the microscope.The serum vascular endothelial growth factor (VEGF) concentration was detected by Elisa Kit. RT-PCR was used to detect the expression of VEGF, nuclear factor E2 (Nrf2), heme oxygenase-1 (HO-1), catalase (CAT), SOD, GPx, interleukin-6(IL-6) and monocyte chemoattractant protein-1 (MCP-1) mRNA.
Results
The adipocyte size, number of vascular/each adipocyte, serum VEGF concentration and VEGF mRNA expression in adipose tissue of high fat diet group were significantly higher than those of normal control group (all P<0.05). EGCG can significantly reduce the above indicators of high fat diet group (all P<0.05). The expression of Nrf2, HO-1, SOD, GPx and CAT mRNA in adipose tissue of EGCG group was significantly higher than those in high fat diet group and normal control group (all P<0.05). The expression of MCP-1 and IL-6 mRNA in adipose tissue of EGCG group was significantly lower than that in high fat diet group (all P<0.05).
Conclusion
EGCG can decrease the production of serum VEGF, vascular density and the expression of VEGF mRNA in white adipose tissue of high fat diet rats, and inhibit the angiogenesis in white adipose tissue possibly due to its up-regulation of Nrf2/HO-1 pathway to increase the expression of antioxidant enzymes (SOD, CAT, GPx), reduce ROS production and decrease the inflammatory response.
Key words:
(-)-Epigallocatechin-3-gallate; High fat diet; White adipose tissue; Angiogenesis; Vascular endothelial growth factor
期刊介绍：
The Chinese Journal of Clinical Nutrition was founded in 1993. It is the first professional academic journal (bimonthly) in my country co-sponsored by the Chinese Medical Association and the Chinese Academy of Medical Sciences to disseminate information on clinical nutrition support, nutrient metabolism, the impact of nutrition support on outcomes and "cost-effectiveness", as well as translational medicine and nutrition research. It is also a professional journal of the Chinese Medical Association's Parenteral and Enteral Nutrition Branch.
The purpose of the Chinese Journal of Clinical Nutrition is to promote the rapid dissemination of knowledge on nutrient metabolism and the rational application of parenteral and enteral nutrition, focusing on the combination of multidisciplinary and multi-regional field investigations and clinical research. It mainly reports on nutritional risk screening related to the indications of parenteral and enteral nutrition support, "cost-effectiveness" research on nutritional drugs, consensus on clinical nutrition, guidelines, expert reviews, randomized controlled studies, cohort studies, glycoprotein and other nutrient metabolism research, systematic evaluation of clinical research, evidence-based case reports, special reviews, case reports and clinical experience exchanges, etc., and has a special column on new technologies related to the field of clinical nutrition and their clinical applications.
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