水稻粒长和品质调控基因bgl的遗传分析和基因定位

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2022-02-25 DOI:10.31742/ijgpb.82.1.3
M. Sunusi, Asadullah Khan, Y. Tsago, Ziyan Chen, C. Shi, Xiaoli Jin
{"title":"水稻粒长和品质调控基因bgl的遗传分析和基因定位","authors":"M. Sunusi, Asadullah Khan, Y. Tsago, Ziyan Chen, C. Shi, Xiaoli Jin","doi":"10.31742/ijgpb.82.1.3","DOIUrl":null,"url":null,"abstract":"Grain size is one of the important agronomic traits that determine rice yield. Map-based cloning of rice grain size-related genes impacts the genetic regulation mechanism of rice yield formation. Here we identified bgl mutant in rice which exhibits increase in grain length and panicle length with the decrease in number of grains per panicle and no observable difference in grain thickness and number of tillers as compared to the wild type (WT). Scanning electron microscopy observation of bgl mutant spikelets hull indicates an upsurge in number of external epidermal cells in a longitudinal way than the WT. Hence, it is likely that the long-grain phenotype was due to increased cell number longitudinally. Starch granule in both bgl and WT were large, sporadically polyhedral and densely packed, indicating no differences, signifying that both bgl mutant and WT have less chalkiness content. Genetic analysis showed that the bgl mutant was controlled by a recessive single gene, and was finely located on chromosome 3 through map-based cloning, within a physical distance of 40kb. Sequencing analysis revealed A to T substitution in the seventeen exons of LOC_Os03g44500 resulted in an amino acid change from Tryptophan (Try) to Phenylalanine (Phe). Taken together bgl mutant has a pleiotropic effect on grain yield and grain quality.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic analysis and gene mapping of bgl, a gene controlling grain length and quality in rice\",\"authors\":\"M. Sunusi, Asadullah Khan, Y. Tsago, Ziyan Chen, C. Shi, Xiaoli Jin\",\"doi\":\"10.31742/ijgpb.82.1.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Grain size is one of the important agronomic traits that determine rice yield. Map-based cloning of rice grain size-related genes impacts the genetic regulation mechanism of rice yield formation. Here we identified bgl mutant in rice which exhibits increase in grain length and panicle length with the decrease in number of grains per panicle and no observable difference in grain thickness and number of tillers as compared to the wild type (WT). Scanning electron microscopy observation of bgl mutant spikelets hull indicates an upsurge in number of external epidermal cells in a longitudinal way than the WT. Hence, it is likely that the long-grain phenotype was due to increased cell number longitudinally. Starch granule in both bgl and WT were large, sporadically polyhedral and densely packed, indicating no differences, signifying that both bgl mutant and WT have less chalkiness content. Genetic analysis showed that the bgl mutant was controlled by a recessive single gene, and was finely located on chromosome 3 through map-based cloning, within a physical distance of 40kb. Sequencing analysis revealed A to T substitution in the seventeen exons of LOC_Os03g44500 resulted in an amino acid change from Tryptophan (Try) to Phenylalanine (Phe). Taken together bgl mutant has a pleiotropic effect on grain yield and grain quality.\",\"PeriodicalId\":13321,\"journal\":{\"name\":\"Indian Journal of Genetics and Plant Breeding\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2022-02-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Genetics and Plant Breeding\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.31742/ijgpb.82.1.3\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/ijgpb.82.1.3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

粒大小是决定水稻产量的重要农艺性状之一。水稻粒大小相关基因的图谱克隆影响水稻产量形成的遗传调控机制。我们在水稻中发现了bgl突变体,该突变体的粒长和穗长增加,每穗粒数减少,但在粒厚和分蘖数上与野生型(WT)没有明显差异。扫描电镜观察bgl突变体小穗壳的外表皮细胞数量在纵向上比WT增加,因此,长粒表型可能是由于纵向上细胞数量增加所致。bgl突变体和WT突变体淀粉粒均较大,偶有多面体,排列致密,无差异,说明bgl突变体和WT的垩白含量均较低。遗传分析表明,bgl突变体由一个隐性单基因控制,通过定位克隆,定位在3号染色体上,物理距离约为40kb。测序分析显示,LOC_Os03g44500的17个外显子的A - T替换导致氨基酸从色氨酸(Try)变为苯丙氨酸(Phe)。综上所述,bgl突变体对籽粒产量和籽粒品质具有多效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Genetic analysis and gene mapping of bgl, a gene controlling grain length and quality in rice
Grain size is one of the important agronomic traits that determine rice yield. Map-based cloning of rice grain size-related genes impacts the genetic regulation mechanism of rice yield formation. Here we identified bgl mutant in rice which exhibits increase in grain length and panicle length with the decrease in number of grains per panicle and no observable difference in grain thickness and number of tillers as compared to the wild type (WT). Scanning electron microscopy observation of bgl mutant spikelets hull indicates an upsurge in number of external epidermal cells in a longitudinal way than the WT. Hence, it is likely that the long-grain phenotype was due to increased cell number longitudinally. Starch granule in both bgl and WT were large, sporadically polyhedral and densely packed, indicating no differences, signifying that both bgl mutant and WT have less chalkiness content. Genetic analysis showed that the bgl mutant was controlled by a recessive single gene, and was finely located on chromosome 3 through map-based cloning, within a physical distance of 40kb. Sequencing analysis revealed A to T substitution in the seventeen exons of LOC_Os03g44500 resulted in an amino acid change from Tryptophan (Try) to Phenylalanine (Phe). Taken together bgl mutant has a pleiotropic effect on grain yield and grain quality.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
期刊最新文献
Assessment of agro-morphological traits and yield-based tolerance indices in sesame (Sesamum indicum L.) genotypes under drought stress Genetic characterization and comparative assessment of response of biparental and multi-parental populations to northern corn leaf blight in maize (Zea mays L.) Identification of efficient learning classifiers for discrimination of coding and non-coding RNAs in plant species Molecular marker assisted confirmation of hybrids in coconut (Cocos nucifera L.) Influence of G x E interaction on heterobeltiosis, combining ability and stability of multiple cross derivatives of cotton (Gossypium hirsutum L.) amenable for HDPS
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1