cGnRH-II基因在三株木犀(Soang、Jepun和Bluesafir)中的鉴定和表达

Q3 Chemistry Molekul Pub Date : 2023-07-10 DOI:10.20884/1.jm.2023.18.2.7616
Rita Eka Rosita, Hamdan Syakuri, A. Nuryanto, E. Hilmi, P. Sukardi, N. A. Prayogo
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引用次数: 0

摘要

地瓜(Osphronemus Gouramy)具有很高的经济价值,易于栽培。目前,根据其繁殖能力,已成功培育出大约六个品系,即鹅(soang,鹅gouramy)、jepon(日本,日本)、蓝宝石、巴黎、巴斯塔(肉鸡)和瓷器。这些能力差异的原因之一是内部因素,这些因素可以通过鉴定和表达每种gouramy菌株的cGnRH基因来看出。cGnRH基因在垂体分泌激素GtH的信号传导中发挥作用。本研究旨在确定序列和基因表达值​​由三个不同年龄水平的古拉米亚特菌株产生。所用的研究方法是探索不同年龄水平(4个月、8个月、12个月)的三个gouramy菌株(soang、jepun、blue sapphire),每个年龄水平取三个Gooramy菌株。本研究经历了器官制备、分离、序列鉴定和cGnRH基因表达测定几个阶段。使用系统发育树分析序列数据,并使用单向方差分析检验分析基因表达。序列结果表明,与蓝蓝宝石菌株相比,soang菌株具有更类似于jepun菌株的序列,由此产生的基因表达表明,三个不同年龄水平的gouramy菌株没有给出不同的结果。
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Identification and Expression of cGnRH-II Gene in Three Strains Osphronemus gouramy (Soang, Jepun and Bluesafir)
Gouramy (Osphronemus gouramy) has very high economic value and is easy to cultivate. Currently there are about six strains that have been successfully cultivated based on their reproductive ability to produce eggs, namely goose (soang, goose gouramy), jepun (japan, japonica), blue sapphire, paris, bastar (broiler) and porcelain. One of the reasons for these differences in ability is internal factors which can be seen through the identification and expression of the cGnRH gene that each of these gouramy strains have. The cGnRH gene functions in signaling the pituitary gland to secrete the hormone GtH. This study aims to identify sequences and gene expression values ​​resulting from three strains of gouramyat different age levels. The research method used was the exploration of three gouramy strains (soang, jepun, blue sapphire) at different age levels (4 months, 8 months, 12 months), and three gouramy strains were taken for each age level. This research was conducted through several stages, namely organ preparation, isolation, sequence identification and measurement of cGnRH gene expression. Sequence data was analyzed using phylogenetic trees and gene expression was analyzed using One Way ANOVA test. The sequence results showed that the soang strain had a sequence that was more similar to the jepun strain than the blue sapphire strain, and the resulting gene expression showed that the three gouramy strains with three different age levels did not give different results.
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来源期刊
Molekul
Molekul Chemistry-Chemistry (all)
CiteScore
1.30
自引率
0.00%
发文量
31
审稿时长
4 weeks
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