Screening of immunogenic proteins from extracellular proteome of C. botulinum type B by immunoproteomic approach

ABSTRACT Botulinum toxin is the highly toxic substance that causes very dangerous neuroparalytic disease called botulism. It is produced by anaerobic and endospore forming bacteria called C. botulinum. In the absence of licensed vaccine against botulism, an immunoproteome-based approach was developed to find immunogenic proteins of C. botulinum type B for development of vaccine against botulism. Extracellular proteins of C. botulinum type B were separated by 2-DE and probed with hyper-immune antisera of extracellular proteins and antisera of live spores of C. botulinum type B to evaluate the reactivity of extracellular proteins by immunoblotting assay. Five proteins reacted with extracellular proteins antisera and one protein reacted with live spores’ antisera. These identified immunogenic proteins will be further evaluated as the vaccine candidates against botulism.