Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta
{"title":"血斑干法测定乙型肝炎病毒DNA的评价及其在实际条件下的稳定性。","authors":"Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta","doi":"10.1016/j.jcvp.2022.100111","DOIUrl":null,"url":null,"abstract":"<div><p>We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R<sup>2</sup>=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.</p></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"2 4","pages":"Article 100111"},"PeriodicalIF":1.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667038022000503/pdfft?md5=c6f1e3cde94c7563efe14a552e824ac0&pid=1-s2.0-S2667038022000503-main.pdf","citationCount":"2","resultStr":"{\"title\":\"Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions\",\"authors\":\"Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta\",\"doi\":\"10.1016/j.jcvp.2022.100111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R<sup>2</sup>=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.</p></div>\",\"PeriodicalId\":73673,\"journal\":{\"name\":\"Journal of clinical virology plus\",\"volume\":\"2 4\",\"pages\":\"Article 100111\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667038022000503/pdfft?md5=c6f1e3cde94c7563efe14a552e824ac0&pid=1-s2.0-S2667038022000503-main.pdf\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical virology plus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667038022000503\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical virology plus","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667038022000503","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions
We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R2=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.
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