扁豆种子电导率试验的充分性

Edvan Costa da Silva, Wagner Menechini, C. Galvão, Luís Augusto Batista de Oliveira, Natália Cássia de Faria Ferreira, Luciana Sabini da Silva
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引用次数: 0

摘要

电导率测试因其执行简单、可重复性、速度快、易于解释结果和成本低而在现有的生理质量测试中脱颖而出。本研究旨在建立一种测试扁豆种子电导率的方法,包括种子的数量和浸泡时间。该实验于2018年在戈亚斯州立大学伊帕梅里校区的多学科实验室进行。使用扁豆种子,储存在同一机构的种子实验室中。所用的实验设计是完全随机的,采用4x5析因方案,四个种子批次(25、50、75和100个种子),在25℃下浸泡五次(3、6、9、12和15小时),四次重复。对初始种子质量进行了表征(含水量、首次发芽计数、发芽百分比、加速老化和1000粒种子重量)。对于电导率测试,使用25、50、75和100个种子的四次重复,在精度为0.001g的数字秤上称重,并放置在容量为200mL的塑料杯中,其中含有75mL蒸馏水。然后,将塑料杯留在BOD室中,在25°C下进行调节,并在电导率仪(DIGIMED DM 31)中浸泡3、6、9、12和15小时后进行测量,结果以μS cm-1 g-1表示。在评估每次重复的种子数量和浸泡时间时存在显著的相互作用。对50个种子进行12小时浸泡的重复使用显示了扁豆种子电导率测试的最佳结果。
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Adequacy of electrical conductivity test for lentil seeds
The electrical conductivity test stands out among the existing physiological quality tests due to its simplicity of execution, repeatability, speed, easy interpretation of results, and low cost. This study aimed to establish a methodology for the test of electric conductivity in lentil seeds (Lens culinaris Medik), involving the seeds’ number of seeds and soaking time. The experiment was conducted at the Multidisciplinary Laboratory of the State University of Goiás, Campus Ipameri, in 2018. Lentil Silvina seeds were used, stored in the seed laboratory of the same institution. The experimental design used was completely randomized, in a 4x5 factorial scheme, with four seed lots (25, 50, 75, and 100 seeds) and five soaking times (3, 6, 9, 12, and 15 hours) at 25 oC, with four replications. The initial seed quality was characterized (water content, first counting of germination, percentage of germination, accelerated aging and 1000-seed weight). For the electrical conductivity test, four repetitions of 25, 50, 75, and 100 seeds each were used, weighed on a digital scale with an accuracy of 0.001 g and placed in plastic cups, with a capacity of 200 mL, containing 75 mL of distilled water. Then, the plastic cups remained in a BOD chamber, adjusted at 25 °C and the measuring was performed after 3, 6, 9, 12, and 15 hours of soaking in a conductivity meter (DIGIMED DM 31), the results being expressed in μS cm -1 g -1 . There was a significant interaction when evaluating the number of seeds per repetition and the soaking time. The use of replicates with 50 seeds subjected to 12 h of soaking shows the best results for the lentil seeds' electrical conductivity test.
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