乙基资产组分Cabai Rawit(Capsicum frutescens L.)有机智能成分的鉴定及抗炎活性测试

Uci Pradina, Ajuk Sapar, Warsidah Warsidah, Endah Sayekti, A. B. Aritonang
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引用次数: 1

摘要

众所周知,辣椒可以治疗组织细胞肿胀,并被认为具有抗炎活性。研究了辣椒的乙酸乙酯部位的抗炎活性,并鉴定了乙酸乙酯部位的有机化合物成分。本研究的阶段包括制备,提取,分配,植物化学试验,使用人类红细胞(HRBC)进行抗炎活性试验,以及鉴定其所含有机化合物的成分。在提取阶段得到的3个部位中,乙酸乙酯部位的抗炎活性最好,浓度为1000 mg/L,为57.77%。采用真空液相色谱法(VLC)对乙酸乙酯部分进行分离,得到4个组合馏分。用GC-MS和FTIR分析了其中一个组合组分,代码EA-4-5,质量为0.188 g。GC-MS分析得到的两个主要化合物为十二烷酸(25.79%)和(-)-榄油内酯(12.86%)。EA-4-5组分的FTIR解析结果显示,吸收波数分别为3234.62 cm-1 (-OH官能团的拉伸振动)、3088.03 cm-1和3028.24 cm-1(官能团的拉伸振动=CH)、2931.8 cm-1和2858.51 cm-1(官能团的应变振动-CH)、1712.79 cm-1(官能团的应变振动-C =O)和1606.7 cm-1(应变振动官能团-C =C)。
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Identifikasi Komponen Senyawa Organik dan Uji Aktivitas Antiinflamasi dari Fraksi Etil Asetat Daun Cabai Rawit (Capsicum frutescens L.)
Cayenne pepper was known to treat swelling of tissue cells and is thought to have anti-inflammatory activity. The aim study is to examine the anti-inflammatory activity of the ethyl acetate fraction of cayenne pepper and identify the components of organic compounds in the ethyl acetate fraction. The stages of this research include preparation, extraction, partitioning, phytochemical tests, anti-inflammatory activity tests using Human Red Blood Cells (HRBC), and identifying the components of organic compounds they contain. Among the three fractions obtained at the extraction stage, the ethyl acetate fraction had the best activity as an anti-inflammatory at a concentration of 1000 mg/L of 57.77%. The fractionation of the ethyl acetate fraction was carried out using Vaccum Liquid Chromatography (VLC), and four combined fractions were obtained. One of the combined fractions, code EA-4-5, with a weight of 0.188 grams, was analyzed using GC-MS and FTIR. The two main compounds obtained from the GC-MS analysis were dodecanoic acid (25.79%) and (-)-Loliolide (12.86%). The results of the FTIR interpretation of the EA-4-5 fraction showed absorption at a wave number of 3234.62 cm-1 (stretching vibration of the -OH functional group), 3088.03 cm-1 and 3028.24 cm-1 (stretching vibration of the functional group =CH), 2931.8 cm-1 and 2858.51 cm-1 (functional group strain vibration –CH), 1712.79 cm-1 (functional group strain vibration –C=O), and 1606.7 cm-1 (strain vibration functional group –C=C).
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