In Vitro Osteogenic Potential of Freeze-Dried Homologous Platelet-Rich Plasma

Introduction: Platelet-rich plasma (PRP) is paid attention for regenerative therapy because it clinically improves neoangiogenesis and periodontal regeneration. PRP can be made in form of homologous PRP (HPRP) from healthy, screened, and habitual blood donors and freeze-dried to increase stored time of HPRP and maintain growth factors. The purpose of this study is to evaluate freeze-dried homologous platelet-rich plasma (FD HPRP) on osteogenesis. Material and Methods: HPRP was taken from the blood bank and then a freeze-drying and γ-radiation process was carried out with doses of 20 and 25 KGy, respectively, for the sterilization. Blood was collected as much as 10 mL and then centrifuged at 700 rpm for 3 minutes to produce injectable platelet-rich fibrin (iPRF) as a positive control. Cell line MG63 were cultured to confluent, and then treated with FD HPRP 20 kGy, FD HPRP 25 kGy, iPRF, and one group non treated as a negative control. Osteocytes were determined based on morphology after hematoxylin staining to see the differentiation process. The data were analyzed using one-way analysis of variance (ANOVA). Results: There was a significant difference in the number of osteocytes between FD HPRP and negative control (P < 0.05). The number of osteocytes FD HPRP 25 was higher than FD HPRP 20. Conclusion: The present results indicate that FD HPRP could support the bone regeneration and used without any preparation like fresh PRP.