γ辐照对BALB/c小鼠模型中布鲁氏菌的保护作用

A. Al-Mariri, Laila Al-Hallab, Rasha Alabras, Heba Kherbik, Marwa Khawajkiah
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引用次数: 0

摘要

目的布鲁氏菌是一种人畜共患病,在世界范围内引起人类波动热和牲畜流产。最近,有人表示,通过辐照研制的疫苗已引起强烈的细胞和体液免疫反应,使这类疫苗非常有效。材料与方法本研究以γ辐照的b - ovis为疫苗,研究其对abortus 544、b - melitensis 16M和b - ovis的保护作用,并研究其体液免疫反应和细胞因子的产生。结果γ辐照后免疫小鼠的体液免疫反应持续8周。在末次免疫4周和8周后,检测到免疫球蛋白G (IgG)、IgG1、IgG2a和IgG2b同型抗体。我们注意到免疫BALB/c小鼠的脾细胞在4周和8周后继续产生肿瘤坏死因子-α、干扰素-γ和白细胞介素(IL)-10,而未观察到IL-4或IL-5的产生。结论BALB/c小鼠对melitensis 16M、B. abortus 544和B. ovis具有诱导保护作用,且本实验室研制的疫苗具有与传统疫苗相似的保护作用。
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Protection against virulent Brucella spp. by gamma-irradiated B. ovis in BALB/c mice model
Purpose Brucella spp. is a zoonosis that causes undulant fever in humans and abortion in livestock worldwide. Lately, it was conveyed that vaccines developed by irradiation have induced a strong cellular and humoral immune response which have made these types of vaccines highly effective. Materials and Methods In this study, we aimed to use the gamma-irradiated B. ovis as a vaccine and to study the humoral immune response and cytokines production in order to evaluate it for protecting mice against B. abortus 544, B. melitensis 16M, and B. ovis. Results The humoral immune response in immunized mice with gamma-irradiated B. ovis showed a lasting for 8 weeks after immunization. Moreover, immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b isotypes antibodies against B. ovis were observed after 4 and 8 weeks of the last immunization. It was noticed that the production of tumor necrosis factor-α, interferon-γ, and interleukin (IL)-10 continued after 4 and 8 weeks by splenocytes from immunized BALB/c mice, while no production of IL-4 or IL-5 was observed. Conclusion Our results indicate that the protection of BALB/c mice against B. melitensis 16M, B. abortus 544, and B. ovis was induced and the developed vaccine at our laboratory could stimulate similar protection to those induced by the traditional vaccine.
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来源期刊
CiteScore
3.70
自引率
3.70%
发文量
29
审稿时长
8 weeks
期刊介绍: Clin Exp Vaccine Res, the official English journal of the Korean Vaccine Society, is an international, peer reviewed, and open-access journal. It covers all areas related to vaccines and vaccination. Clin Exp Vaccine Res publishes editorials, review articles, special articles, original articles, case reports, brief communications, and correspondences covering a wide range of clinical and experimental subjects including vaccines and vaccination for human and animals against infectious diseases caused by viruses, bacteria, parasites and tumor. The scope of the journal is to disseminate information that may contribute to elaborate vaccine development and vaccination strategies targeting infectious diseases and tumors in human and animals. Relevant topics range from experimental approaches to (pre)clinical trials for the vaccine research based on, but not limited to, basic laboratory, translational, and (pre)clinical investigations, epidemiology of infectious diseases and progression of all aspects in the health related issues. It is published printed and open accessed online issues (https://ecevr.org) two times per year in 31 January and 31 July. Clin Exp Vaccine Res is linked to many international databases and is made freely available to institutions and individuals worldwide
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