荷斯坦奶牛酮症生化变化监测方法学

V. Marutsova
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引用次数: 0

摘要

摘要本研究的目的是建立亚临床和临床形式酮症荷斯坦奶牛的生化和病理学变化。本研究共包括47头泌乳期为1至4期的荷斯坦奶牛。从所有动物身上采集血样,用于测定β-羟基丁酸(ВНВА,mmol/l)、非酯化脂肪酸(NEFA,mmol/l)、葡萄糖(Gl,mmol/l)、天冬氨酸转氨酶(ASAT,U/l)、丙氨酸转氨酶(ALAT,U/l)和总胆红素(Tb,µmol/l)。根据奶牛的生理状况,将其分为三组:怀孕、刚产仔和哺乳期。根据血液BHBA水平,将三组奶牛分为健康奶牛(对照组,n=24,BHBA2.6 mmol/l)。病理学研究是在对诊断为临床酮症的奶牛进行尸检后进行的。与对照组相比,三组SCK奶牛的NEFA血液水平在统计学上显著升高,而与对照组和SCK相比,CK–奶牛的血液NEFA水平降低。与对照组相比,SCK和CK酮症奶牛的葡萄糖水平降低,而ASAT、ALAT和Tb活性升高。组织学研究显示,患有临床酮症的奶牛肝细胞有核溶解、核破裂、细胞空泡化,以及肝脏和肾脏的坏死变化和脂肪营养不良。
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Methodology for monitoring biochemical changes in Holstein cows with ketosis
Abstract. The purpose of the present study was to establish the biochemical and pathohistological changes in Holstein cows with subclinical and clinical form of ketosis. A total of 47 Holstein cows from 1st to 4th lactation were included in the study. Blood samples were obtained from all animals for determination of β-hydroxybutyrate (ВНВА, mmol/l), non-esterified fatty acids (NEFA, mmol/l), glucose (Gl, mmol/l), aspartate aminotransferase (ASAT, U/l), alanine aminotransferase (ALAT, U/l) and total bilirubin (Tb, µmol/l). The cows were divided into three groups depending on their physiological condition: pregnant, recently calved and lactating. The cows from the three groups were classified as healthy (control, n=24, BHBA<1.2 mmol/L), affected with subclinical ketosis (SCK, n=15, BHBA from 1.2 to 2.6 mmol/l) and with clinical ketosis (CK, n=8, BHBA>2.6 mmol/l) depending on their blood BHBA levels. The pathohistological investigations were done after autopsy of cows diagnosed with clinical ketosis. The blood levels of NEFA in cows of the three groups with SCK were statistically significantly elevated vs control groups, while in cows with CK – decreased, vs both controls and SCK. The levels of glucose decreased, while the activities of ASAT, ALAT and Tb levels were increased in cows with SCK and CK ketosis vs controls. Histological studies revealed karyolysis, karyorrhexis, cellular vacuolation in hepatocytes, as well as necrotic changes and fatty dystrophy of the liver and kidneys in cows with clinical ketosis.
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