肠球菌分离株毒力决定因素及耐药性的研究

IF 2.1 Q2 MEDICINE, GENERAL & INTERNAL BioMedicine-Taiwan Pub Date : 2023-07-01 DOI:10.51248/.v43i3.1160
Reena Rajan, K. Dhandapani
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引用次数: 0

摘要

介绍和目的:毒力因素的存在可能会导致肠球菌在医疗环境中的持久性增加,增加住院患者的定植能力,从而导致感染的传播。本研究旨在检测肠球菌临床菌株中存在的毒力特征,并确定其毒力因素与对各种抗生素的敏感性之间的关系。材料与方法:采用常规方法和Vitek 2自动化方法对临床分离的肠球菌进行种级鉴定,采用纸片扩散法和最低抑制浓度法进行药敏试验。采用表型法检测溶血素、明胶酶和生物膜的存在。结果:708株尿液分离株中粪肠球菌39株(5.51%)产生生物膜,粪肠球菌和durans肠球菌各3株(0.42%)产生生物膜。从尿液和脓液中分别检出342只(48.30%)和9只(18.75%)粪肠球菌产生β溶血素。在所研究的分离株中,9/774(1.16%)分离株在β -溶血素、明胶酶和生物膜生产方面呈阳性。9株(100.00%)均对青霉素、高剂量庆大霉素、环丙沙星和左氧氟沙星耐药。结论:肠球菌中描述的毒力因子使这些生物能够在患者组织中定植,增加对抗菌药物的耐药性并加重感染结果。
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A study on virulence determinants and antimicrobial resistance among Enterococci isolates
Introduction and Aim: Presence of virulence factors may cause increased persistence of Enterococci in the healthcare environment, increase ability to colonise inpatients and thereby result in the transmission of infection. The present study was performed to detect the presence of virulence traits among the clinical strains of Enterococci and to determine its association between virulence factors   and susceptibility to various antibiotics.   Materials and Methods: Clinical isolates of Enterococci were identified to species level by conventional method and Vitek 2 automated method and subjected to antibiotic susceptibility testing using disk diffusion and Minimum Inhibitory Concentration (MIC) method. Presence of hemolysin, gelatinase and biofilm was detected by phenotypic method.   Results: Out of 708 isolates from urine 39 (5.51%) Enterococcus faecalis, and 3 isolates each of Enterococcus faecium (0.42 %) and Enterococcus durans (0.42 %) were biofilm producers. Beta hemolysin production was detected in 342 (48.30%) E. faecalis obtained from urine and 9/48 (18.75 %) from pus. Out of the isolates studied, 9/774 (1.16 %) isolates were found to be positive for beta-hemolysin production, gelatinase and biofilm production. All the 9 (100.00 %) isolates were resistant to penicillin, high level gentamicin, ciprofloxacin and levofloxacin.   Conclusion: Virulence factors described in Enterococci enable these organisms to colonise patient tissue, increase resistance to antimicrobial agents and aggravate infection outcome.
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来源期刊
BioMedicine-Taiwan
BioMedicine-Taiwan MEDICINE, GENERAL & INTERNAL-
CiteScore
2.80
自引率
5.90%
发文量
21
审稿时长
24 weeks
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