鉴定与MERS-CoV RNA基因组5 '端结合的宿主因子

IF 2.1 4区 医学 Q3 VIROLOGY Future Virology Pub Date : 2023-05-22 DOI:10.2217/fvl-2023-0070
Bader Y. Alhatlani, Waleed Aljabr, F. Alhamlan, A. Almatroudi, M. Azam, M. Alsaleem, K. Allemailem
{"title":"鉴定与MERS-CoV RNA基因组5 '端结合的宿主因子","authors":"Bader Y. Alhatlani, Waleed Aljabr, F. Alhamlan, A. Almatroudi, M. Azam, M. Alsaleem, K. Allemailem","doi":"10.2217/fvl-2023-0070","DOIUrl":null,"url":null,"abstract":"Aim: The aim of this study was to identify host factors that interact with the 5′ end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses. We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of host factors that bind to the 5′ end of the MERS-CoV RNA genome\",\"authors\":\"Bader Y. Alhatlani, Waleed Aljabr, F. Alhamlan, A. Almatroudi, M. Azam, M. Alsaleem, K. Allemailem\",\"doi\":\"10.2217/fvl-2023-0070\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim: The aim of this study was to identify host factors that interact with the 5′ end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses. We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.\",\"PeriodicalId\":12505,\"journal\":{\"name\":\"Future Virology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-05-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2217/fvl-2023-0070\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Virology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2217/fvl-2023-0070","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:本研究的目的是鉴定与MERS-CoV RNA基因组5′端相互作用的宿主因子。材料与方法:采用RNA亲和色谱法和质谱分析法对Vero E6细胞中宿主因子的结合进行鉴定。结果:在未感染的Vero E6细胞中,共鉴定出59个与MERS-CoV RNA基因组结合的宿主因子。大多数已鉴定的细胞蛋白先前被报道与其他RNA病毒的基因组相互作用。我们使用蛋白质印迹法验证了我们的质谱结果。结论:这些数据增强了我们对冠状病毒的RNA-宿主相互作用的了解,可以作为开发针对MERS-CoV的抗病毒疗法的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Identification of host factors that bind to the 5′ end of the MERS-CoV RNA genome
Aim: The aim of this study was to identify host factors that interact with the 5′ end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses. We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Future Virology
Future Virology 医学-病毒学
CiteScore
4.00
自引率
3.20%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.
期刊最新文献
Trends and perspectives in tuberculosis and HIV co-infection studies over the past three decades Human RSVA-ON1, the only genotype present during 2019–2020 winter season in Riyadh, Saudi Arabia: a retrospective study Rosmarinic acid inhibits Rift Valley fever virus: in vitro, computational and analytical studies Plain language summary of the efficacy and safety of bepirovirsen in patients with chronic hepatitis B infection Expression and significance of IL-17A and IL-22 in children with infectious mononucleosis complicated with liver damage
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1