鉴定与MERS-CoV RNA基因组5 '端结合的宿主因子

IF 2.1 4区 医学 Q3 VIROLOGY Future Virology Pub Date : 2023-05-22 DOI:10.2217/fvl-2023-0070
Bader Y. Alhatlani, Waleed Aljabr, F. Alhamlan, A. Almatroudi, M. Azam, M. Alsaleem, K. Allemailem
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引用次数: 0

摘要

目的:本研究的目的是鉴定与MERS-CoV RNA基因组5′端相互作用的宿主因子。材料与方法:采用RNA亲和色谱法和质谱分析法对Vero E6细胞中宿主因子的结合进行鉴定。结果:在未感染的Vero E6细胞中,共鉴定出59个与MERS-CoV RNA基因组结合的宿主因子。大多数已鉴定的细胞蛋白先前被报道与其他RNA病毒的基因组相互作用。我们使用蛋白质印迹法验证了我们的质谱结果。结论:这些数据增强了我们对冠状病毒的RNA-宿主相互作用的了解,可以作为开发针对MERS-CoV的抗病毒疗法的靶点。
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Identification of host factors that bind to the 5′ end of the MERS-CoV RNA genome
Aim: The aim of this study was to identify host factors that interact with the 5′ end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses. We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.
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来源期刊
Future Virology
Future Virology 医学-病毒学
CiteScore
4.00
自引率
3.20%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.
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