RGB定量方法在分析化学教学中的应用

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY Periodico Tche Quimica Pub Date : 2022-10-11 DOI:10.52571/ptq.v19.n42.2022.01_veloso_pgs_01_12.pdf
Pedro Henrique F Veloso, V. D. M. Sacramento, V. Royo
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引用次数: 0

摘要

背景:在本科学习中使用分析化学的替代教学方法对教师来说是一个挑战,因为大多数方法都需要高成本的设备和功能分析技能。这项工作介绍了使用RGB,一种数字介质的附加色系统,作为量化样品中给定分析物的方法。目的:分析含有不同浓度咖啡的不同样品,使用两种技术进行比较:1)分光光度法和2)RGB,然后进行计算以确定未知浓度。方法:制备8个样品,其中6个含有规定浓度的咖啡,1个为白色,1个没有规定浓度。方法1使用分光光度计读取420nm波长下的吸光度以读取黑色素,在方法2中使用RGB,将桶定位在发射相应波长的光的数字屏幕前面。Microsoft Powerpoint用于收集RGB和Microsoft Excel数据进行数据处理。结果:通过分析设备获得的数据以及通过RGB获得的数据能够估计未知的咖啡浓度,这两种技术估计的浓度都在3%到4%之间,直线方程和r²都能够估计与样品浓度相对应的值。讨论:近几十年来,人们一直在研究RGB技术,主要用于比色分析,因为它可以以一种方便的方式提供研究或实验室实验。让学生更接近分光光度法,促进科学发展,并阐述将技术作为分析工具的假设。结论:实验实践促进了研究的发展,指导教师和学生使用新技术和开展旨在教学的新活动,这项工作为使用易于获取和低成本的工具和方法开辟了一个范围。
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USE OF RGB AS A QUANTITATIVE METHOD APPLIED TO THE TEACHING OF ANALYTICAL CHEMISTRY
Background: The use of alternatives for teaching analytical chemistry in undergraduate studies has been a challenge for teachers since most methods require high-cost equipment and functional analytical skills. This work presents the use of RGB, an additive color system n digital media, as a method of quantifying of a given analyte in samples. Aim: To analyze different samples containing different concentrations of coffee, comparing them using two techniques: 1) spectrophotometry and 2) RGB, and then perform the calculations for the determination of an unknown concentration. Methods: Eight samples were prepared, six with defined concentrations of coffee, one in white, and one without a defined concentration. Method 1 used the spectrophotometer to read the absorbance at 420 nm wavelength for melanoidin reading, in method 2 the RGB was used, positioning buckets in front of a digital screen emitting light at the corresponding wavelength. Microsoft Powerpoint was used to collect RGB and Microsoft Excel data for data processing. Results: The data obtained through the analytical equipment, as well as those obtained via RGB, were able to estimate the unknown concentration of coffee, both techniques estimated a concentration between 3 and 4%, both with the equation of the line and r² capable of estimating values corresponding to the concentrations of the samples. Discussion: The RGB technique has been studied in recent decades, mainly for colorimetric analyses, since it makes the study or laboratory experiments available in an accessible way. Bringing the student closer to the spectrophotometric technique, promoting scientific development, and elaborating the hypothesis about using technology as an analysis tool. Conclusions: Experimental practices promote the development of research, which guide both teachers and students in the use of new technologies and in the development of new activities aimed at teaching, this work in question opens a range for the use of tools and methods of easy access and low cost.
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Periodico Tche Quimica
Periodico Tche Quimica CHEMISTRY, MULTIDISCIPLINARY-
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期刊介绍: The Journal publishes original research papers, review articles, short communications (scientific publications), book reviews, forum articles, announcements or letters as well as interviews. Researchers from all countries are invited to publish on its pages.
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