问题信息

Q1 Biochemistry, Genetics and Molecular Biology Current Protocols in Protein Science Pub Date : 2020-09-11 DOI:10.1002/cpps.91

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摘要

封面:在Welch等人(http://doi.org/10.1002/cpps.113)中，图像显示了捕获和释放(CaRe)方法的示意图。1、粗提物。2、粗提取物与目标捕获剂(TCA)混合。TCA通过结合和交联来捕获靶标(凝集素或糖蛋白)。4、离心后，将含有全部杂质的上清液丢弃。只有目标- tca复合物留在试管中。5、在配合物上加入单价配体(ML)。ML溶解复合物并释放靶标。7、通过过滤将释放的靶标与TCA分离。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Issue Information

Cover: In Welch et al. (http://doi.org/10.1002/cpps.113), the image shows schematic presentation of the capture and release (CaRe) method. 1, crude extracts. 2, crude extracts mixed with a target capturing agent (TCA). 3, TCA captures target (lectin or glycoprotein) by binding and cross-linking the target. 4, after centrifugation, the supernatant containing all the impurities is discarded. Only the target-TCA complex remains in the test tube. 5, monovalent ligand (ML) is added to the complex. 6, ML dissolves the complex and releases the target. 7, the released target is separated from the TCA by filtration.

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来源期刊

Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.

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