锡尔特省血根头蜱犬埃利希体的分子鉴定

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES Acta Veterinaria-Beograd Pub Date : 2021-09-01 DOI:10.2478/acve-2021-0022

B. Çelik, Adnan Ayan, A. Yılmaz, Ö. Çelik, Ö. Kilinç, Ö. Ayan

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引用次数: 1

摘要

摘要本研究采用PCR法对采集的犬埃利希体阳性蜱进行16S rRNA基因片段测序。从土耳其锡尔特省共60只狗身上采集蜱虫。采集蜱虫250只，进行形态调查。所有蜱属均为血鼻蜱(s.l)。PCR法检测到38只蜱(15.2%)的埃利希体DNA。通过序列分析获得的犬E. canis菌株与美国德克萨斯州(MH620194)、印度(KX766395)和埃及(MG564254)菌株完全相同。因此，本研究已从锡尔特省收集的狗身上的血鼠蜱中确定了一种人畜共患病原体。

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Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province

Abstract This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.