Krüppel同源基因1调控二化螟潜在靶转录因子基因的鉴定

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Journal of Entomological Science Pub Date : 2023-06-26 DOI:10.18474/JES22-39
Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu
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引用次数: 0

摘要

摘要幼激素(JH)是一种主要的内分泌激素,介导昆虫的发育、变态和繁殖。它直接与耐甲氧平受体结合,并招募异二聚体伴侣形成JH-受体复合物,然后激活JH诱导基因Krüppel同源物1(Kr-h1)。有证据表明,该基因是介导生理和生化过程的下游因子;然而,Kr-h1的功能机制在很大程度上是未知的。以经济上重要的水稻害虫二化螟(Lepidoptera:Crambidae)为模型,我们结合RNA干扰(RNAi)、高通量RNA测序和实时定量聚合酶链式反应(RT-qPCR)来鉴定受Kr-h1调控的候选转录因子(TF)基因。Krh1的RNAi敲除鉴定了由Kr-h1调节的锌指蛋白、ZBTB、THAP、PAX、MYB、HSF、同源框、HMG、CSD、碱性螺旋-环-螺旋、STAT、RHD和MBD家族。RT-qPCR证实了这些假定的转录因子的转录水平,并表明敲低Kr-h1可以诱导或抑制这些蛋白在二化螟中的表达。这些结果为深入研究Kr-h1在二化螟和其他昆虫中调节的转录因子提供了所需的基本信息。
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Identification of Potential Target Transcription Factor Genes Regulated by Krüppel Homolog 1 in Chilo suppressalis (Lepidoptera: Crambidae)
Abstract Juvenile hormone (JH) is a major endocrine hormone that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor and recruits a heterodimer partner to form the JH–receptor complex that then activates a JH-inducible gene known as the Krüppel homolog 1 (Kr-h1). There is evidence that this gene is a downstream factor mediating both physiological and biochemical processes; however, the functional mechanism of Kr-h1 is largely unknown. Using the economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) as a model, we used a combination of RNA interference (RNAi), high-throughput RNA sequencing, and real-time quantitative polymerase chain reaction (RT-qPCR) to identify candidate transcription factor (TF) genes that are regulated by Kr-h1. RNAi knockdown of Krh1 identified the Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, and MBD families as regulated by Kr-h1. RT-qPCR confirmed the transcription levels of these putative TFs and indicated that knockdown of Kr-h1 can induce or suppress the expression of these proteins in C. suppressalis. These results provide the basic information required for in-depth research on the TFs regulated by Kr-h1 in C. suppressalis and other insects.
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来源期刊
CiteScore
1.20
自引率
11.10%
发文量
40
审稿时长
>12 weeks
期刊介绍: The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research
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