Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu
{"title":"Krüppel同源基因1调控二化螟潜在靶转录因子基因的鉴定","authors":"Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu","doi":"10.18474/JES22-39","DOIUrl":null,"url":null,"abstract":"Abstract Juvenile hormone (JH) is a major endocrine hormone that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor and recruits a heterodimer partner to form the JH–receptor complex that then activates a JH-inducible gene known as the Krüppel homolog 1 (Kr-h1). There is evidence that this gene is a downstream factor mediating both physiological and biochemical processes; however, the functional mechanism of Kr-h1 is largely unknown. Using the economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) as a model, we used a combination of RNA interference (RNAi), high-throughput RNA sequencing, and real-time quantitative polymerase chain reaction (RT-qPCR) to identify candidate transcription factor (TF) genes that are regulated by Kr-h1. RNAi knockdown of Krh1 identified the Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, and MBD families as regulated by Kr-h1. RT-qPCR confirmed the transcription levels of these putative TFs and indicated that knockdown of Kr-h1 can induce or suppress the expression of these proteins in C. suppressalis. These results provide the basic information required for in-depth research on the TFs regulated by Kr-h1 in C. suppressalis and other insects.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of Potential Target Transcription Factor Genes Regulated by Krüppel Homolog 1 in Chilo suppressalis (Lepidoptera: Crambidae)\",\"authors\":\"Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu\",\"doi\":\"10.18474/JES22-39\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Juvenile hormone (JH) is a major endocrine hormone that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor and recruits a heterodimer partner to form the JH–receptor complex that then activates a JH-inducible gene known as the Krüppel homolog 1 (Kr-h1). There is evidence that this gene is a downstream factor mediating both physiological and biochemical processes; however, the functional mechanism of Kr-h1 is largely unknown. Using the economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) as a model, we used a combination of RNA interference (RNAi), high-throughput RNA sequencing, and real-time quantitative polymerase chain reaction (RT-qPCR) to identify candidate transcription factor (TF) genes that are regulated by Kr-h1. RNAi knockdown of Krh1 identified the Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, and MBD families as regulated by Kr-h1. RT-qPCR confirmed the transcription levels of these putative TFs and indicated that knockdown of Kr-h1 can induce or suppress the expression of these proteins in C. suppressalis. These results provide the basic information required for in-depth research on the TFs regulated by Kr-h1 in C. suppressalis and other insects.\",\"PeriodicalId\":15765,\"journal\":{\"name\":\"Journal of Entomological Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Entomological Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.18474/JES22-39\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Entomological Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.18474/JES22-39","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Identification of Potential Target Transcription Factor Genes Regulated by Krüppel Homolog 1 in Chilo suppressalis (Lepidoptera: Crambidae)
Abstract Juvenile hormone (JH) is a major endocrine hormone that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor and recruits a heterodimer partner to form the JH–receptor complex that then activates a JH-inducible gene known as the Krüppel homolog 1 (Kr-h1). There is evidence that this gene is a downstream factor mediating both physiological and biochemical processes; however, the functional mechanism of Kr-h1 is largely unknown. Using the economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) as a model, we used a combination of RNA interference (RNAi), high-throughput RNA sequencing, and real-time quantitative polymerase chain reaction (RT-qPCR) to identify candidate transcription factor (TF) genes that are regulated by Kr-h1. RNAi knockdown of Krh1 identified the Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, and MBD families as regulated by Kr-h1. RT-qPCR confirmed the transcription levels of these putative TFs and indicated that knockdown of Kr-h1 can induce or suppress the expression of these proteins in C. suppressalis. These results provide the basic information required for in-depth research on the TFs regulated by Kr-h1 in C. suppressalis and other insects.
期刊介绍:
The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research