{"title":"真核翻译起始因子3亚基D与GRP78相互作用,通过Warburg效应促进非霍奇金淋巴瘤的临床靶点和瘤前基因","authors":"Zhong Kong, Yong Liu, Jing Zhu","doi":"10.1166/jbt.2023.3261","DOIUrl":null,"url":null,"abstract":"Non-Hodgkin’s lymphoma (NHL) is one highly heterogeneous tumor originating from the lymphatic system. Its morbidity rate shows a gradually increasing trend year by year. The present study determined that the possible function and mechanisms of EIF3D on cell proliferation of NHL.\n A total of 24 patients with NHL were obtained at Aerospace Center Hospital. In patients with NHL, mRNA and protein expression of EIF3D was up-regulated. Patients with low EIF3D possessed better survival rate. EIF3D stimulated cell proliferation and the number of Edu cells through the activation\n of Warburg effect in vitro model of NHLNHL. Sh-EIF3D diminished NHL cell proliferation and the number of Edu cells through the inactivation of Warburg effect in vitro model of NHL. Then, we found that EIF3D reduced GRP78 protein ubiquitination to induce GRP78/Akt proteins, and\n si-EIF3D promote GRP78 protein ubiquitination to suppress GRP78/Akt proteins in vitro model of NHL. Our results indicate that EIF3D promote NHL cell proliferation throughWarburg effect by the inhibition of GRP78 protein ubiquitination, suggesting that it may prove to be one clinical\n target and pre-tumor gene for NHL.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Eukaryotic Translation Initiation Factor 3 Subunit D is One Clinical Target and Pre-Tumor Gene for Non Hodgkin Lymphoma to Promote Cell Proliferation Through Warburg Effect by Interacting with GRP78\",\"authors\":\"Zhong Kong, Yong Liu, Jing Zhu\",\"doi\":\"10.1166/jbt.2023.3261\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Non-Hodgkin’s lymphoma (NHL) is one highly heterogeneous tumor originating from the lymphatic system. Its morbidity rate shows a gradually increasing trend year by year. The present study determined that the possible function and mechanisms of EIF3D on cell proliferation of NHL.\\n A total of 24 patients with NHL were obtained at Aerospace Center Hospital. In patients with NHL, mRNA and protein expression of EIF3D was up-regulated. Patients with low EIF3D possessed better survival rate. EIF3D stimulated cell proliferation and the number of Edu cells through the activation\\n of Warburg effect in vitro model of NHLNHL. Sh-EIF3D diminished NHL cell proliferation and the number of Edu cells through the inactivation of Warburg effect in vitro model of NHL. Then, we found that EIF3D reduced GRP78 protein ubiquitination to induce GRP78/Akt proteins, and\\n si-EIF3D promote GRP78 protein ubiquitination to suppress GRP78/Akt proteins in vitro model of NHL. Our results indicate that EIF3D promote NHL cell proliferation throughWarburg effect by the inhibition of GRP78 protein ubiquitination, suggesting that it may prove to be one clinical\\n target and pre-tumor gene for NHL.\",\"PeriodicalId\":15300,\"journal\":{\"name\":\"Journal of Biomaterials and Tissue Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1166/jbt.2023.3261\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials and Tissue Engineering","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1166/jbt.2023.3261","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Eukaryotic Translation Initiation Factor 3 Subunit D is One Clinical Target and Pre-Tumor Gene for Non Hodgkin Lymphoma to Promote Cell Proliferation Through Warburg Effect by Interacting with GRP78
Non-Hodgkin’s lymphoma (NHL) is one highly heterogeneous tumor originating from the lymphatic system. Its morbidity rate shows a gradually increasing trend year by year. The present study determined that the possible function and mechanisms of EIF3D on cell proliferation of NHL.
A total of 24 patients with NHL were obtained at Aerospace Center Hospital. In patients with NHL, mRNA and protein expression of EIF3D was up-regulated. Patients with low EIF3D possessed better survival rate. EIF3D stimulated cell proliferation and the number of Edu cells through the activation
of Warburg effect in vitro model of NHLNHL. Sh-EIF3D diminished NHL cell proliferation and the number of Edu cells through the inactivation of Warburg effect in vitro model of NHL. Then, we found that EIF3D reduced GRP78 protein ubiquitination to induce GRP78/Akt proteins, and
si-EIF3D promote GRP78 protein ubiquitination to suppress GRP78/Akt proteins in vitro model of NHL. Our results indicate that EIF3D promote NHL cell proliferation throughWarburg effect by the inhibition of GRP78 protein ubiquitination, suggesting that it may prove to be one clinical
target and pre-tumor gene for NHL.