木质纤维素支架固定化酶构建新型模块化生物反应器

IF 9.1 Q1 ENGINEERING, CHEMICAL Green Chemical Engineering Pub Date : 2023-03-01 DOI:10.1016/j.gce.2022.03.001
Zhihong Zhang , Mengchen Jin , Guiru Chen , Jiandu Lei , Luying Wang , Jun Ge
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引用次数: 2

摘要

模块化生物反应器可以为构建复杂的多步途径提供灵活的平台,这可能是最大化反应和克服多酶系统复杂性的解决方案。在此,我们选择木材衍生的纤维素支架作为酶固定化的载体,并构建了模块化生物反应器。在去除木材中的木质素后,制备纤维素支架,然后进行柠檬酸功能化,并加入戊二醛,最终使酶交联。将辣根过氧化物酶(HRP)、葡萄糖氧化酶(GOD)和过氧化氢酶(CAT)三种酶分别固定化,使固定化酶的量超过40mg/g。从柠檬酸中引入羧基有助于酶在载体表面的快速吸附,固定化酶具有~65%的表达活性。利用固定化酶构建了模块化生物反应器。利用固定化HRP模块,反应器显示出期望的催化性能,苯酚降解率>;90%。此外,pH调节可以发生在生物反应器中,用于保存酶活性和中和酸产物。在GOD/CAT模块化生物反应器中,调节pH值的级联反应可以实现95%的葡萄糖酸钠产率,并表现出5个操作循环的良好可重复使用性。
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Enzymes immobilized in wood-derived cellulose scaffold for constructing a novel modular bioreactor

Modular bioreactors can provide a flexible platform for constructing complex multi-step pathways, which may be a solution for maximizing reactions and overcoming the complexity of multi-enzyme systems. Here, we selected wood-derived cellulose scaffold as a support for enzyme immobilization and constructed the modular bioreactor. Cellulose scaffold was prepared after removing lignin from wood, followed by citric acid functionalization and the addition of glutaraldehyde finally allowed the cross-linking of enzymes. Three enzymes, horseradish peroxidase (HRP), glucose oxidase (GOD), and catalase (CAT), were separately immobilized, resulting in the immobilized enzyme amount to over 40 mg/g. The introduction of carboxyl groups from citric acid facilitated the rapid enzyme adsorption on the support surface and immobilized enzymes possess ∼65% expressed activity. Modular bioreactors were constructed by using the immobilized enzymes. With the immobilized HRP module, reactor showed desired catalytic performance with the phenol degradation rate of > 90%. Also, a pH regulation can occur in the bioreactors for preserving enzyme activities and neutralizing acid products. In the GOD/CAT modular bioreactor, the cascade reaction with adjusting pH values can achieve a 95% yield of sodium gluconate and exhibit a favorable reusability of 5 operation cycles.

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来源期刊
Green Chemical Engineering
Green Chemical Engineering Process Chemistry and Technology, Catalysis, Filtration and Separation
CiteScore
11.60
自引率
0.00%
发文量
58
审稿时长
51 days
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