细胞衰老在培养和体内的定量分析

Q1 Health Professions Current Protocols in Cytometry Pub Date : 2017-01-05 DOI:10.1002/cpcy.16
Jing Zhao, Heike Fuhrmann-Stroissnigg, Aditi U. Gurkar, Rafael R. Flores, Akaitz Dorronsoro, Donna B. Stolz, Claudette M. St. Croix, Laura J. Niedernhofer, Paul D. Robbins
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引用次数: 13

摘要

细胞衰老是指正常分裂细胞在各种应激作用下的不可逆生长停滞。细胞衰老是由重复细胞分裂引起的端粒缩短引起的,这会引起DNA损伤反应,以及基因毒性、氧化和炎症应激。强烈的有丝分裂信号,如癌基因激活,也会驱使细胞进入衰老状态。衰老细胞表达一种特定的基因亚群,称为衰老相关分泌表型(SASP),包括促炎因子、生长因子和基质金属蛋白酶,它们共同促进非细胞自主的继发性衰老。清除随着年龄增长而积累的衰老细胞可以改善健康寿命,这表明细胞衰老是衰老过程的一个促成因素。因此,需要一种方法来鉴定和量化细胞衰老,无论是在培养细胞还是在体内。本文描述了最具特征和广泛使用的衰老测定方法,从细胞形态学和衰老相关的β-半乳糖苷酶(SA-βgal)染色到核生物标志物、SASP和肿瘤抑制因子水平的改变。©2017 by John Wiley &儿子,Inc。
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Quantitative Analysis of Cellular Senescence in Culture and In Vivo

Cellular senescence refers to the irreversible growth arrest of normally dividing cells in response to various types of stress. Cellular senescence is induced by telomere shortening due to repeated cell division, which causes a DNA damage response, as well as genotoxic, oxidative, and inflammatory stress. Strong mitogenic signaling, such as oncogene activation, also drives cells into a senescent state. Senescent cells express a specific subset of genes, termed the senescence-associated secretory phenotype (SASP), including pro-inflammatory factors, growth factors, and matrix metalloproteinases, which together promote non-cell autonomous, secondary senescence. Clearance of senescent cells that accumulate with age improves health span, implicating cellular senescence as a contributing factor to the aging process. Thus, there is a need for methods to identify and quantify cellular senescence, both in cultured cells and in vivo. Here, methods for the most well-characterized and widely used senescent assays are described, from cell morphology and senescence-associated β-galactosidase (SA-βgal) staining to nuclear biomarkers, SASP, and altered levels of tumor suppressors. © 2017 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Cytometry
Current Protocols in Cytometry Health Professions-Medical Laboratory Technology
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期刊介绍: Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.
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