Gas Chromatography Mass Spectrometry (GC-MS) Profiling of Arjunarishta

Gas chromatography with mass spectrometric detectors are used to separate different analytes present in a mixture and identify the same. It can also be used for quantification of the analytes. Sample preparation is essential for GC-MS analysis to obtain desired analytes. Aim of the study: The present study is pursued to profile the phytochemicals present in Arjunarishta, a medicated alcoholic preparation through Dichloromethane (DCM) and Ethyl acetate (EA). Materials and Methods: A sample obtained from a GMP-certified pharmacy was subjected to liquid-liquid extraction using DCM and EA, which was examined with GC-MS. Results: The analysis yielded 19 and 20 phytochemicals in DCM and EA respectively. Presumably, due to a small difference in the polarity of the solvents used, nine phytochemicals such as Ethyl gallate, Catechols etc., were found in both extracts. Conclusion: 30 unique phytochemicals were established in two different solvent extracts of the same sample.