基于LC-MS的Nigella sativa提取物代谢产物分析、体外抗氧化和体内抗高血压活性

IF 4 Q2 FOOD SCIENCE & TECHNOLOGY eFood Pub Date : 2023-07-28 DOI:10.1002/efd2.107
Amit Kumar Shrivastava, Laxmi Shrestha, Buddhi Raj Pokhrel, Bishal Joshi, Gopal Lamichhane, Bojana Vidović, Niranjan Koirala
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引用次数: 0

摘要

本研究的目的是鉴定黑草水提物中存在的生物活性成分,并评价其抗氧化和抗高脂血症的活性。采用LC-MS/MS分析方法测定了苜蓿提取物中不同生物活性成分的含量。采用经验证的福林比色法和氯化铝比色法分别测定总酚和类黄酮含量。并对其体外抗氧化和体内抗高脂血症活性进行了评价。初步的植物化学筛选表明,水提物中存在生物碱、类黄酮、酚类、苷类和氨基酸。通过LC-MS/MS分析鉴定了水提物的生物活性成分。对DPPH、H2O2和OH自由基的清除能力最强,IC50值分别为11.916±2.828、30.294±13.790和12.048±2.828µg/mL。高剂量(800 mg/kg)提取液可显著降低Wistar大鼠血中总胆固醇(TC) 71.76±6.91 mg/dL, TG 83.6±8.09 mg/dL,低密度脂蛋白(LDL-c) 33.86±6.05 mg/dL,极低密度脂蛋白(VLDL-c) 16.72±1.61 mg/dL。然而,在治疗28天后,与hfd诱导的对照组大鼠(11.76±1.14 mg/dL)相比,HDL-C水平显著提高(21.18±1.80 mg/dL)。在相同剂量下,动物体重由对照组的184.4±10.24 g降至162.6±16.40 g。苜蓿的水提取物被发现是一种有效的抗氧化剂和降血脂剂的天然来源。这种活性归因于其中存在多种生物活性化合物。
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LC-MS based metabolite profiling, in-vitro antioxidant and in-vivo antihyperlipidemic activity of Nigella sativa extract

The aim of this study was to identify the bioactive phytoconstituents present in the aqueous extract of Nigella sativa and also, to evaluate the antioxidant and antihyperlipidemic activity in Wistar rats. The LC-MS/MS analysis was assessed for the determination of different bioactive compounds present in N. sativa extract. Total phenolic and flavonoid content were determined by using validated Folin-Ciocalteu and Aluminum chloride colorimetric methods, respectively. The in-vitro antioxidant and in-vivo antihyperlipidemic activity in Wistar rats were also evaluated. Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, phenols, glycosides, and amino acids in the aqueous extract. The bioactive compounds of the aqueous extract were identified through LC-MS/MS analysis. The in-vitro antioxidant activity of N. sativa showed the highest free radical scavenging capacity in DPPH, H2O2, and OH radical scavenging assays with IC50 values 11.916 ± 2.828, 30.294 ± 13.790, and 12.048 ± 2.828 µg/mL, respectively. Evaluation of antihyperlipidemic activity of extract in Wistar rats showed that a high dose (800 mg/kg) of extract significantly decreased total cholesterol (TC) 71.76 ± 6.91 mg/dL, TG 83.6 ± 8.09 mg/dL, low-density lipoproteins (LDL-c) 33.86 ± 6.05 mg/dL, very low-density lipoproteins (VLDL-c) 16.72 ± 1.61 mg/dL level in blood. However, the HDL-C level was significantly improved (21.18 ± 1.80 mg/dL) as compared to HFD-induced control rats (11.76 ± 1.14 mg/dL) after 28 days of treatment. Also, at the same dose, animal body weight was also decreased to 162.6 ± 16.40 g compared with control 184.4 ± 10.24 g. The aqueous extract of N. sativa was found to be an effective natural source of antioxidant and hypolipidemic agents. This activity was attributed to the presence of diverse bioactive compounds in it.

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来源期刊
eFood
eFood food research-
CiteScore
6.00
自引率
0.00%
发文量
44
期刊介绍: eFood is the official journal of the International Association of Dietetic Nutrition and Safety (IADNS) which eFood aims to cover all aspects of food science and technology. The journal’s mission is to advance and disseminate knowledge of food science, and to promote and foster research into the chemistry, nutrition and safety of food worldwide, by supporting open dissemination and lively discourse about a wide range of the most important topics in global food and health. The Editors welcome original research articles, comprehensive reviews, mini review, highlights, news, short reports, perspectives and correspondences on both experimental work and policy management in relation to food chemistry, nutrition, food health and safety, etc. Research areas covered in the journal include, but are not limited to, the following: ● Food chemistry ● Nutrition ● Food safety ● Food and health ● Food technology and sustainability ● Food processing ● Sensory and consumer science ● Food microbiology ● Food toxicology ● Food packaging ● Food security ● Healthy foods ● Super foods ● Food science (general)
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