Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández
{"title":"临床细胞学在奶牛肝脂质沉积诊断中的应用","authors":"Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández","doi":"10.21753/VMOA.5.1.386","DOIUrl":null,"url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physical, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multiparous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"5 1","pages":"1-14"},"PeriodicalIF":0.2000,"publicationDate":"2018-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.1.386","citationCount":"1","resultStr":"{\"title\":\"Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle\",\"authors\":\"Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández\",\"doi\":\"10.21753/VMOA.5.1.386\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physical, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multiparous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.\",\"PeriodicalId\":49387,\"journal\":{\"name\":\"Veterinaria Mexico\",\"volume\":\"5 1\",\"pages\":\"1-14\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2018-03-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.21753/VMOA.5.1.386\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinaria Mexico\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.21753/VMOA.5.1.386\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria Mexico","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21753/VMOA.5.1.386","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Veterinary","Score":null,"Total":0}
Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physical, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multiparous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.
期刊介绍:
Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges.
This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).