临床细胞学在奶牛肝脂质沉积诊断中的应用

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2018-03-16 DOI:10.21753/VMOA.5.1.386
Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández
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引用次数: 1

摘要

Veterinaria Mexico OA ISSN:2448-6760引用如下:Rodriguez Fierros FL,Quiroz Rocha G,Nunez Ochoa L,Aparicio Cecilio A,Salgado Hernandez EG。临床细胞学在诊断奶牛肝脂血症中的实际应用。兽医墨西哥OA。2018年;5(1)。doi:10.21753/vmoa.5.1.386.肝脂血症是一种常见于奶牛的代谢紊乱。它在奶牛场的临床评估很复杂,因为它是基于物理、化学和生物测试的。本研究评估了肝细胞学在奶牛中的实际应用,并表明该方法不会影响动物的整体健康和乳制品生产率。在过渡期的三个不同时间(产仔时以及产后10天和21天),从25头随机选择的多胎荷斯坦奶牛身上采集细胞和血液样本。测定红细胞压积、血浆蛋白、纤维蛋白原、白细胞总数和差异计数,以及总蛋白、白蛋白、谷氨酸脱氢酶、三酰甘油酯、胆固醇和s-羟基丁酸酯的值。根据检测到的脂肪浸润程度对细胞学结果进行分类,并比较每个采样期的血液学和代谢结果。记录每个采样阶段的健康状况和每日牛奶产量。获得的结果表明,动物没有表现出显著的临床炎症或乳制品产量下降,这可以被认为是继发于细胞学采样。因此,该技术被认为是诊断奶牛肝脂质沉积症的一种可行的替代方法。图1。在棘突下方约15至20cm的右侧第11肋间从牛肝脏抽吸细胞。
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Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physi­cal, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multipa­rous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
自引率
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>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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