洋葱提取物的植物化学筛选及其抗氧化活性

M. El-Saied, S. El‐Saadany, H. Hefnawy, A. El-Sayed
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引用次数: 1

摘要

对洋葱不同溶剂提取物进行了植物化学筛选和抗氧化活性研究。对提取物进行植物化学成分的各种化学测试。用Folin-Ciocalteu法测定总酚含量,并用DPPH·assay、FRAP和ABTS通过“体外”自由基清除活性测定其抗氧化活性。本研究的植物化学筛选表明存在类固醇、类黄酮、生物碱、皂苷和儿茶素单宁。水乙醇提取物的平均总多酚含量显著高于己烷、乙酸乙酯和二氯甲烷提取物(P<0.05)。在使用(DPPH、ABTS和FRAP)时,植物提取物的有效性顺序(IC50)是有效的抑制剂是水乙醇提取物,其次是二氯甲烷、乙酸乙酯,而己烷提取物最少。这表明洋葱有机溶剂提取物,特别是水乙醇提取物,可能是天然抗氧化剂的有效来源,可用于治疗与氧化应激相关的疾病
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PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ONION(Allium cepa L.) EXTRACTS
Phytochemical screening and antioxidant activities in different solvent extracts of onion was carried out. The extracts were subjected to various chemical Test for phytochemical constituents. Total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through «in vitro» radical scavenging activity using DPPH· assay, FRAP and ABTS. The phytochemical screening of this study indicated the presence of steroids, flavonoids, alkaloids, saponnins, and catechic tannins. The average total polyphenol content of hydroethanolic extracts was significantly (P<0.05) higher than in the hexane, ethyl acetate and dichloromethane extracts. The order of effectiveness (IC50) of the plant extracts the potent inhibitors was hydroethanolic extract, followed by Dichloromethane, ethyl acetate while the least was the hexane extract, When using (DPPH, ABTS and FRAP). This shows that onion organic solvent extracts especially the hydroethanolic extracts may be a potent source of natural antioxidant and can be used in the management of diseases associated with oxidative stress is justified
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