ANTITUBERCULOSIS EFFECTIVENESS OF ENDOPHYTIC ANDALAS MUTANT BACTERIA PRODUCS INDUCED BY UV LIGHT WILL BE CONDUCTED

Indonesia is the 3rd largest country in the world in cases of tuberculosis (TB) infection. Infections caused by the bacteria Mycobacterium Tuberculosis have claimed 100,000 lives per year. This death rate is expected to continue to increase due to resistance to Antituberculosis Drugs (OAT). One strategy to overcome the increasing number of resistance of M. tuberculosis bacteria to OAT is to find new and better antituberculosis active compounds. This study aims to determine the potential of Andalas Endophytic Bacteria mutated by UV light on antituberculosis activity. This research is descriptive research. The research was conducted at the Research Laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University. The mutation process is carried out as a form of increasing the production of active compounds. Natural anti microbial compounds can be obtained through the fermentation process. The active compounds from the fermentation products were extracted using methanol and aquadest as solvents. Testing against M. tuberculosis using the Mycobacterium Growth Indicator Tube (MGIT) 960 System as a medium for sensitivity testing. The fermented supernatant with absolute concentration and extract of each solvent with a concentration of 75% were mixed into the MGIT 960 tube. The results showed that the length of time UV irradiation could reduce the number of endophytic bacterial cells of Andalas isolate ATB 10-6. UV light also causes changes in the morphology of mutant bacterial colonies. UV irradiation can produce mutant isolates that have better antifungal activity, where of the 8 mutant bacteria produced, 7 mutants have better antifungal activity than the wild type, and the growth of mutant bacteria is faster than the wild type.