Effect of Naringin on myocardial potency of Resveratrol against ischemia reperfusion induced myocardial toxicity in rat

Objective

Resveratrol (RES) is a well known cardioprotective phytoconstituent, but the poor bioavailability provides further scope for research to improve its therapeutic efficacy. The present study was designed to address this challenge by combining with bio-enhancer like Naringin (NAR) in the prevention of ischemia reperfusion injury (IRI) induced myocardial toxicity in rats.

Methods

Rats (n = 8) were treated with RES (20 mg/kg, p.o.) alone and combination of NAR (15 mg/kg, p.o.) and RES (20 mg/kg, p.o.) for 30 days. Twenty four hour after last treatment Ischemia reperfusion injury was induced by modified Lagendorff apparatus, and the effect of different treatments was evaluated by percentage recovery in terms of heart rate and developed tension, biomarkers, heart tissue antioxidant levels and a histopathological examination. The Influence of NAR on the pharmacokinetics of RES was studied by HPLC. Results were assessed by one‑way analysis of variance followed by Tukey–Karmer multiple comparison test.

Results

Combination of RES and NAR demonstrated significant (P < 0.01) restoration of biomarker, antioxidant, tension and heart rate compared to RES alone treated group. Significant (P < 0.01) increase in bioavailability and half life, along with significant (P < 0.001) decrease in clearance was observed for RES in combination group compared to RES alone treated group.

Conclusion

The combination of RES and NAR exhibited profound protection compared to RES alone treated group against IRI induced myocardial toxicity. Findings of pharmacokinetic interaction support the results of a pharmacodynamic interaction.