{"title":"没食子酸和水杨酸对三角褐指藻生长和生化参数影响的比较评价","authors":"N. N. Kovalev, S. Leskova, E. V. Mikheev","doi":"10.15578/iaj.18.1.2023.37-44","DOIUrl":null,"url":null,"abstract":"The research of the effect of gibberellic (0.4 - 3.8x 10-8 mol.L-1) and salicylic (0.4 - 3.8 x 10-5 mol.L-1) acids, in a wide range of their concentrations, on the growth indicators and biochemical composition of the cumulative culture of the microalgae Phaeodactylum tricornutum was carried out. It was determined that salicylic acid in a concentration of 0.4 x 10-5 mol stimulated cell growth by 184.6%, and gibberellic acid at a concentration of 0.39 x 10-8 mol by 181%, compared to the control. The effect of gibberellic acid during the experiment was expressed in the inhibition of protein accumulation in the culture, compared with the control. The use of salicylic acid led to a greater accumulation of protein in the culture than when using gibberellic acid. It was shown that salicylic acid had a positive effect on the accumulation of carbohydrates on day 9 and gibberellic acid on day 14 of culture. Gibberellic acid had no effect on the accumulation of lipids in the culture of microalgae. Under the action of salicylic acid for 14 days of cultivation, the lipid content increased by 18.5%, compared with the control. There were no quantitative differences in the content of chlorophyll when using two phytohormones. In this study, the optimal concentrations of gibberellic and salicylic acids for linear growth rate and the highest production of protein and carbohydrates for Phaeodactylum tricornutum were determined. Position, depending on the stage of microalgae growth, is noted.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"COMPARATIVE ASSESSMENT OF THE EFFECT OF GIBBERELLIC AND SALICYLIC ACIDS ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF Phaeodactylum tricornutum\",\"authors\":\"N. N. Kovalev, S. Leskova, E. V. Mikheev\",\"doi\":\"10.15578/iaj.18.1.2023.37-44\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The research of the effect of gibberellic (0.4 - 3.8x 10-8 mol.L-1) and salicylic (0.4 - 3.8 x 10-5 mol.L-1) acids, in a wide range of their concentrations, on the growth indicators and biochemical composition of the cumulative culture of the microalgae Phaeodactylum tricornutum was carried out. It was determined that salicylic acid in a concentration of 0.4 x 10-5 mol stimulated cell growth by 184.6%, and gibberellic acid at a concentration of 0.39 x 10-8 mol by 181%, compared to the control. The effect of gibberellic acid during the experiment was expressed in the inhibition of protein accumulation in the culture, compared with the control. The use of salicylic acid led to a greater accumulation of protein in the culture than when using gibberellic acid. It was shown that salicylic acid had a positive effect on the accumulation of carbohydrates on day 9 and gibberellic acid on day 14 of culture. Gibberellic acid had no effect on the accumulation of lipids in the culture of microalgae. Under the action of salicylic acid for 14 days of cultivation, the lipid content increased by 18.5%, compared with the control. There were no quantitative differences in the content of chlorophyll when using two phytohormones. In this study, the optimal concentrations of gibberellic and salicylic acids for linear growth rate and the highest production of protein and carbohydrates for Phaeodactylum tricornutum were determined. Position, depending on the stage of microalgae growth, is noted.\",\"PeriodicalId\":36566,\"journal\":{\"name\":\"Indonesian Aquaculture Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indonesian Aquaculture Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15578/iaj.18.1.2023.37-44\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Aquaculture Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15578/iaj.18.1.2023.37-44","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 0
摘要
研究了不同浓度的赤霉素(0.4-3.8x10-8 mol.L-1)和水杨酸(0.4-3.8 x10-5 mol.L-1。经测定,与对照相比,0.4 x 10-5 mol浓度的水杨酸刺激细胞生长184.6%,0.39 x 10-8 mol浓度的赤霉酸刺激细胞生长181%。与对照相比,赤霉素在实验期间的作用表现为抑制培养物中的蛋白质积累。与使用赤霉酸相比,水杨酸的使用导致培养物中蛋白质的积累更多。结果表明,水杨酸对培养第9天的碳水化合物积累和第14天的赤霉酸积累有积极影响。赤霉素对微藻培养物中脂质的积累没有影响。在水杨酸的作用下,培养14天,脂质含量比对照增加18.5%。当使用两种植物激素时,叶绿素含量没有定量差异。在本研究中,确定了赤霉素和水杨酸对三角褐指藻线性生长速率和最高蛋白质和碳水化合物产量的最佳浓度。根据微藻生长的阶段,注意位置。
COMPARATIVE ASSESSMENT OF THE EFFECT OF GIBBERELLIC AND SALICYLIC ACIDS ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF Phaeodactylum tricornutum
The research of the effect of gibberellic (0.4 - 3.8x 10-8 mol.L-1) and salicylic (0.4 - 3.8 x 10-5 mol.L-1) acids, in a wide range of their concentrations, on the growth indicators and biochemical composition of the cumulative culture of the microalgae Phaeodactylum tricornutum was carried out. It was determined that salicylic acid in a concentration of 0.4 x 10-5 mol stimulated cell growth by 184.6%, and gibberellic acid at a concentration of 0.39 x 10-8 mol by 181%, compared to the control. The effect of gibberellic acid during the experiment was expressed in the inhibition of protein accumulation in the culture, compared with the control. The use of salicylic acid led to a greater accumulation of protein in the culture than when using gibberellic acid. It was shown that salicylic acid had a positive effect on the accumulation of carbohydrates on day 9 and gibberellic acid on day 14 of culture. Gibberellic acid had no effect on the accumulation of lipids in the culture of microalgae. Under the action of salicylic acid for 14 days of cultivation, the lipid content increased by 18.5%, compared with the control. There were no quantitative differences in the content of chlorophyll when using two phytohormones. In this study, the optimal concentrations of gibberellic and salicylic acids for linear growth rate and the highest production of protein and carbohydrates for Phaeodactylum tricornutum were determined. Position, depending on the stage of microalgae growth, is noted.