S. Waters, S. Ledford, A. Wacker, Sonali Verma, Bianca Serda, J. McKaig, J. Varelas, Patrick Nicoll, K. Venkateswaran, David J. Smith
{"title":"长时间测序显示,在国际空间站上长时间飞行暴露后,短小芽孢杆菌SAFR-32基因组变异和腺苷甲基化的发生率增加","authors":"S. Waters, S. Ledford, A. Wacker, Sonali Verma, Bianca Serda, J. McKaig, J. Varelas, Patrick Nicoll, K. Venkateswaran, David J. Smith","doi":"10.1017/s1473550421000343","DOIUrl":null,"url":null,"abstract":"\n Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years. The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome. Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures. Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.","PeriodicalId":13879,"journal":{"name":"International Journal of Astrobiology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Long-read sequencing reveals increased occurrence of genomic variants and adenosine methylation in Bacillus pumilus SAFR-032 after long-duration flight exposure onboard the International Space Station\",\"authors\":\"S. Waters, S. Ledford, A. Wacker, Sonali Verma, Bianca Serda, J. McKaig, J. Varelas, Patrick Nicoll, K. Venkateswaran, David J. Smith\",\"doi\":\"10.1017/s1473550421000343\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years. The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome. Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures. Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.\",\"PeriodicalId\":13879,\"journal\":{\"name\":\"International Journal of Astrobiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2021-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Astrobiology\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1017/s1473550421000343\",\"RegionNum\":4,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ASTRONOMY & ASTROPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Astrobiology","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1017/s1473550421000343","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ASTRONOMY & ASTROPHYSICS","Score":null,"Total":0}
Long-read sequencing reveals increased occurrence of genomic variants and adenosine methylation in Bacillus pumilus SAFR-032 after long-duration flight exposure onboard the International Space Station
Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years. The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome. Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures. Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.
期刊介绍:
International Journal of Astrobiology is the peer-reviewed forum for practitioners in this exciting interdisciplinary field. Coverage includes cosmic prebiotic chemistry, planetary evolution, the search for planetary systems and habitable zones, extremophile biology and experimental simulation of extraterrestrial environments, Mars as an abode of life, life detection in our solar system and beyond, the search for extraterrestrial intelligence, the history of the science of astrobiology, as well as societal and educational aspects of astrobiology. Occasionally an issue of the journal is devoted to the keynote plenary research papers from an international meeting. A notable feature of the journal is the global distribution of its authors.