L. Sahoo, P. Swain, Ajmal Hussan, A. Mohapatra, J. Sundaray
{"title":"DNA条形码描述了印度西孟加拉邦东加尔各答湿地Pterygoplichthys属入侵南美吸盘嘴甲鲶鱼的单一物种身份","authors":"L. Sahoo, P. Swain, Ajmal Hussan, A. Mohapatra, J. Sundaray","doi":"10.21077/ijf.2022.69.4.124310-15","DOIUrl":null,"url":null,"abstract":"Invasive suckermouth armoured sailfin catfishes of Pterygoplichthys genus is a threat to natural fish biodiversity. Weattempted to identify suckermouth armoured sailfin catfishes, Pterygoplichthys spp. which have successfully invadedand established in the East Kolkata Wetland, using morphological and molecular techniques. Based on the colourationpattern/spot on the belly region they were categorised into three groups viz., Pterygoplichthys pardalis, Pterygoplichthysdisjunctivus and an intermediate group. In total, fin clips from 32 individuals were collected from the above three groupsand a portion of mitochondrial cytochrome oxidase subunit-I (COI) gene was sequenced using Sanger’s method. Out of the32 individuals, good quality sequences were obtained for 30 individuals. Surprisingly, mtDNA COI gene sequence analysisrevealed that a sequence length of 581 bp was identical among all individuals. Species level barcode records from BOLDdatabase revealed the identity of specimens in all categories as P. pardalis. Results of the study suggest that Pterygoplichthysestablished in East Kolkata Wetland might have originated from a single reproducing entity.\nKeywords: Cytochrome oxidase subunit1, East Kolkata Wetland, Mitochondrial DNA, Pterygoplichthys, Suckermouthsailfin catfish","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":" ","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA barcoding delineates the identity of invasive South American sucker mouth armoured catfishes of genus Pterygoplichthys of East Kolkata Wetland, West Bengal, India as single species\",\"authors\":\"L. Sahoo, P. Swain, Ajmal Hussan, A. Mohapatra, J. Sundaray\",\"doi\":\"10.21077/ijf.2022.69.4.124310-15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Invasive suckermouth armoured sailfin catfishes of Pterygoplichthys genus is a threat to natural fish biodiversity. Weattempted to identify suckermouth armoured sailfin catfishes, Pterygoplichthys spp. which have successfully invadedand established in the East Kolkata Wetland, using morphological and molecular techniques. Based on the colourationpattern/spot on the belly region they were categorised into three groups viz., Pterygoplichthys pardalis, Pterygoplichthysdisjunctivus and an intermediate group. In total, fin clips from 32 individuals were collected from the above three groupsand a portion of mitochondrial cytochrome oxidase subunit-I (COI) gene was sequenced using Sanger’s method. Out of the32 individuals, good quality sequences were obtained for 30 individuals. Surprisingly, mtDNA COI gene sequence analysisrevealed that a sequence length of 581 bp was identical among all individuals. Species level barcode records from BOLDdatabase revealed the identity of specimens in all categories as P. pardalis. Results of the study suggest that Pterygoplichthysestablished in East Kolkata Wetland might have originated from a single reproducing entity.\\nKeywords: Cytochrome oxidase subunit1, East Kolkata Wetland, Mitochondrial DNA, Pterygoplichthys, Suckermouthsailfin catfish\",\"PeriodicalId\":50372,\"journal\":{\"name\":\"Indian Journal of Fisheries\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2022-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Fisheries\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.21077/ijf.2022.69.4.124310-15\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Fisheries","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21077/ijf.2022.69.4.124310-15","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
DNA barcoding delineates the identity of invasive South American sucker mouth armoured catfishes of genus Pterygoplichthys of East Kolkata Wetland, West Bengal, India as single species
Invasive suckermouth armoured sailfin catfishes of Pterygoplichthys genus is a threat to natural fish biodiversity. Weattempted to identify suckermouth armoured sailfin catfishes, Pterygoplichthys spp. which have successfully invadedand established in the East Kolkata Wetland, using morphological and molecular techniques. Based on the colourationpattern/spot on the belly region they were categorised into three groups viz., Pterygoplichthys pardalis, Pterygoplichthysdisjunctivus and an intermediate group. In total, fin clips from 32 individuals were collected from the above three groupsand a portion of mitochondrial cytochrome oxidase subunit-I (COI) gene was sequenced using Sanger’s method. Out of the32 individuals, good quality sequences were obtained for 30 individuals. Surprisingly, mtDNA COI gene sequence analysisrevealed that a sequence length of 581 bp was identical among all individuals. Species level barcode records from BOLDdatabase revealed the identity of specimens in all categories as P. pardalis. Results of the study suggest that Pterygoplichthysestablished in East Kolkata Wetland might have originated from a single reproducing entity.
Keywords: Cytochrome oxidase subunit1, East Kolkata Wetland, Mitochondrial DNA, Pterygoplichthys, Suckermouthsailfin catfish
期刊介绍:
Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR), New Delhi. Original contributions in the field of Fish and fisheries science are considered for publication in the Journal. The material submitted must be unpublished and not under consideration for publication elsewhere.
Papers based on research which kills or damages any species, regarded as thratened/ endangered by IUCN crieteria or is as such listed in the Red Data Book appropriate to the geographic area concerned, will not be accepted by the Journal, unless the work has clear conservation objectives.