计划和进行蛛网膜下腔出血啮齿动物模型实验的动物福利方面。

IF 3.6 4区 医学 Q3 CELL BIOLOGY Cellular and Molecular Neurobiology Pub Date : 2023-11-01 Epub Date: 2023-10-20 DOI:10.1007/s10571-023-01418-5
Katrin Becker
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引用次数: 0

摘要

蛛网膜下腔出血是一种急性危及生命的脑血管疾病,具有较高的社会经济影响。最常见的原因是脑内动脉瘤破裂,伴随着脑内压力、脑灌注压力以及脑血流量的突然变化。由于动脉瘤是自发破裂的,只有在住院后一段时间才能对患者的这些参数进行监测。为了研究发作后立即大脑灌注的变化,必须建立动物模型。根据欧盟第2010/63/EU号指令,本文阐述了动物项目提案中必须包含的要点,以保护用于科学目的的动物,并在此深入了解使用啮齿动物模型研究蛛网膜下腔出血后脑灌注的动物福利方面。它比较了最常用的啮齿动物模型——血管内穿孔模型和交叉前和单或双大池注射模型——的手术、模型特征、优点和缺点。还介绍了麻醉讨论、动物麻醉前后处理建议、根据欧盟指令2010/63/EU评估动物在手术过程中遭受的痛苦的严重程度,以及从伦理角度权衡这些体内模型在实验研究中的使用。总之,蛛网膜下腔出血的啮齿动物模型显示出病理生理特征,包括与临床情况相似的脑灌注变化,使该模型适合研究出血后遗症。目前的一个问题是模型的标准化程度低,因此强烈建议根据ARRIVE指南进行报告。蛛网膜下腔出血啮齿动物模型的动物福利方面。就蛛网膜下腔出血后大脑灌注研究的啮齿动物模型的手术和模型特征进行了比较,并提出了3R措施。讨论了麻醉,并就麻醉前后的处理提出了建议。根据2010/63/EU对痛苦的严重程度进行评估,并对这些体内模型的使用进行道德权衡。
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Animal Welfare Aspects in Planning and Conducting Experiments on Rodent Models of Subarachnoid Hemorrhage.

Subarachnoid hemorrhage is an acute life-threatening cerebrovascular disease with high socio-economic impact. The most frequent cause, the rupture of an intracerebral aneurysm, is accompanied by abrupt changes in intracerebral pressure, cerebral perfusion pressure and, consequently, cerebral blood flow. As aneurysms rupture spontaneously, monitoring of these parameters in patients is only possible with a time delay, upon hospitalization. To study alterations in cerebral perfusion immediately upon ictus, animal models are mandatory. This article addresses the points necessarily to be included in an animal project proposal according to EU directive 2010/63/EU for the protection of animals used for scientific purposes and herewith offers an insight into animal welfare aspects of using rodent models for the investigation of cerebral perfusion after subarachnoid hemorrhage. It compares surgeries, model characteristics, advantages, and drawbacks of the most-frequently used rodent models-the endovascular perforation model and the prechiasmatic and single or double cisterna magna injection model. The topics of discussing anesthesia, advice on peri- and postanesthetic handling of animals, assessing the severity of suffering the animals undergo during the procedure according to EU directive 2010/63/EU and weighing the use of these in vivo models for experimental research ethically are also presented. In conclusion, rodent models of subarachnoid hemorrhage display pathophysiological characteristics, including changes of cerebral perfusion similar to the clinical situation, rendering the models suited to study the sequelae of the bleeding. A current problem is low standardization of the models, wherefore reporting according to the ARRIVE guidelines is highly recommended. Animal welfare aspects of rodent models of subarachnoid hemorrhage. Rodent models for investigation of cerebral perfusion after subarachnoid hemorrhage are compared regarding surgeries and model characteristics, and 3R measures are suggested. Anesthesia is discussed, and advice given on peri- and postanesthetic handling. Severity of suffering according to 2010/63/EU is assessed and use of these in vivo models weighed ethically.

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来源期刊
CiteScore
7.70
自引率
0.00%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Cellular and Molecular Neurobiology publishes original research concerned with the analysis of neuronal and brain function at the cellular and subcellular levels. The journal offers timely, peer-reviewed articles that describe anatomic, genetic, physiologic, pharmacologic, and biochemical approaches to the study of neuronal function and the analysis of elementary mechanisms. Studies are presented on isolated mammalian tissues and intact animals, with investigations aimed at the molecular mechanisms or neuronal responses at the level of single cells. Cellular and Molecular Neurobiology also presents studies of the effects of neurons on other organ systems, such as analysis of the electrical or biochemical response to neurotransmitters or neurohormones on smooth muscle or gland cells.
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