不同L1反转录转座子盘绕线圈的共同表达可以导致它们的纠缠。

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY Mobile DNA Pub Date : 2023-10-20 DOI:10.1186/s13100-023-00303-8
Nikola A Mizgier, Charlie E Jones, Anthony V Furano
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引用次数: 0

摘要

L1(LINE1)非LTR逆转录转座子是普遍存在的基因组寄生虫,是人类中的主要转座子~ 灵长类动物1亿年的活动。L1在生殖系细胞和早期胚胎中复制,导致遗传多样性和缺陷,但在一些体细胞干细胞、肿瘤和衰老过程中可能具有活性。L1编码两种逆转录转座所必需的蛋白质:ORF2p,一种含有核酸内切酶结构域的逆转录酶,和ORF1p,一个卷曲的螺旋介导的同源三聚体,起核酸伴侣的作用。这两种蛋白质都含有高度保守的结构域,并优先结合其编码转录物形成L1核糖核蛋白(RNP),该蛋白介导逆转录转座。然而,盘绕的螺旋体周期性地经历了大量氨基酸置换,以至于给定的L1家族可以同时表达其盘绕的序列不同的多个ORF1。在这里,我们表明,当从单独的载体中共同表达时,这种不同的ORF1p序列可以成为纠缠形成的异源三聚体,并推测卷曲线圈纠缠如何影响卷曲线圈的进化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Co-expression of distinct L1 retrotransposon coiled coils can lead to their entanglement.

L1 (LINE1) non-LTR retrotransposons are ubiquitous genomic parasites and the dominant transposable element in humans having generated about 40% of their genomic DNA during their ~ 100 million years (Myr) of activity in primates. L1 replicates in germ line cells and early embryos, causing genetic diversity and defects, but can be active in some somatic stem cells, tumors and during aging. L1 encodes two proteins essential for retrotransposition: ORF2p, a reverse transcriptase that contains an endonuclease domain, and ORF1p, a coiled coil mediated homo trimer, which functions as a nucleic acid chaperone. Both proteins contain highly conserved domains and preferentially bind their encoding transcript to form an L1 ribonucleoprotein (RNP), which mediates retrotransposition. However, the coiled coil has periodically undergone episodes of substantial amino acid replacement to the extent that a given L1 family can concurrently express multiple ORF1s that differ in the sequence of their coiled coils. Here we show that such distinct ORF1p sequences can become entangled forming heterotrimers when co-expressed from separate vectors and speculate on how coiled coil entanglement could affect coiled coil evolution.

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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
期刊最新文献
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