斑马鱼(Danio rerio)循环水系统中人工植物消毒方法的评价。

Christina T Camson, Jack A Palillo, Logan A Fehrenbach, Raphael A Malbrue
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摘要

在生物医学研究设施中使用人工植物作为斑马鱼(Danio rerio)的环境富集物已被证明可以为动物福利和护理提供好处。尽管人工植物对斑马鱼的福利有好处,但由于担心疾病传播,以及缺乏关于水槽之间有效消毒做法的指导,一些研究机构对将其纳入日常饲养实践犹豫不决。关于如何对人造植物进行充分消毒的公开信息有限,这引发了人们对其在再循环水系统中的水箱之间重复使用的担忧。对这些物品进行适当的卫生和消毒对于防止疾病在系统中传播至关重要。我们评估了2种消毒方法─一台商用实验室玻璃器皿洗碗机和一台环氧乙烷(ETO)消毒器─通过在消毒过程前后使用人工植物的ATP检测和细菌培养。在研究开始前,将植物放置在2个独立再循环系统(每个系统2500至3000条鱼)的污水坑中2周。消毒前检测到高ATP水平和各种细菌。商用实验室玻璃器皿洗碗机和ETO消毒器都显著降低了ATP水平,并完全根除了治疗前存在的活细菌。本研究证明了对斑马鱼设施中的人工植物进行消毒的两种有效方法。
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Evaluation of Disinfection Methods for Artificial Plants in Zebrafish (Danio rerio) Recirculating Water Systems.

The use of artificial plants as environmental enrichment for zebrafish (Danio rerio) in biomedical research facilities has been shown to provide benefits in animal welfare and care. Despite the benefits of artificial plants to zebrafish welfare, some research facilities are hesitant to incorporate them into their routine husbandry practices due to concerns about disease transmission and a lack of guidance on effective disinfection practices between tanks. Limited published information is available on how to adequately disinfect artificial plants, which creates concerns regarding their reuse between tanks in recirculating water systems. Proper sanitation and disinfection of these items is crucial to preventing the spread of disease in the system. We evaluated 2 disinfection methods- a commercial-grade laboratory glassware dishwasher and an ethylene oxide (ETO) sterilizer-by using ATP detection and bacterial culture of the artificial plants before and after the disinfection process. Plants were placed in the dirty sump of 2 separate recirculating systems (2,500 to 3,000 fish per system) for 2 wk before the start of the study. High ATP levels and various bacterial organisms were detected prior to disinfection. The commercial-grade labo- ratory glassware dishwasher and ETO sterilizer both significantly reduced ATP levels and resulted in complete eradication of live bacteria that were present before treatment. This study demonstrates 2 effective methods for disinfecting artificial plants in zebrafish facilities.

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American Society of Laboratory Animal Practitioners Position Statement: Handling and Physical Restraint of Research Animals. American Society of Laboratory Animal Practitioners Position Statement: Definition of Animal Welfare. Effect of Novel High-fat Diet Feeding Methods on Food Wastage, Weight Gain, Hair Coat Grease Accumulation, and Scratching Behavior in C57BL/6NCrl Mice. Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin. American Society of Laboratory Animal Practitioners Position Statement: Animal Care Principles.
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