高糖低纤维日粮小鼠盲肠菌群的影响及其对食糜乙酸大分子水提物的抗氧化和免疫调节作用

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-03-22 DOI:10.1016/j.fhfh.2023.100132
Gayang Lee , Takashi Kuda , Yuko Midorikawa , Makoto Nishizawa , Takashi Yamagishi , Ayaka Nakamura , Hajime Takahashi
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引用次数: 0

摘要

大量的日本糖精(SjH)作为培养过程中未使用的副产品被丢弃。在本研究中,我们从SjH中分离出一种功能性褐藻糖胶提取物(FE),作为一种含水乙酸大分子(>;3kDa)级分。FE中的主要化合物是褐藻糖胶和酚类化合物(根皮藤素)。FE在体外表现出显著的超氧化物阴离子自由基清除能力,在大肠杆菌O111缺乏脂多糖(LPS)的情况下促进RAW264.7细胞的一氧化氮(NO)分泌,并在LPS存在的情况下对NO分泌产生抑制作用。给小鼠喂食不含纤维(NF)、0.5%FE(FL)或1.0%FE(FH)的高蔗糖饮食14天。与NF组小鼠的脾脏组织相比,FL和FH喂养的小鼠的促炎细胞因子如白细胞介素(IL)-1β、IL-6和肿瘤坏死因子-α受到抑制。FH对干扰素-γ有抑制作用。盲肠微生物群使用16S rDNA(V4)扩增子测序进行分析。异杆菌和类酸性拟杆菌在FL和FH喂养的小鼠中大量存在。相反,在FH喂养的小鼠中,脱硫弧菌科(与炎症和肠道微生态失调相关的微生物)和普雷沃菌科样细菌的丰度降低。这些结果表明,SjH FE是一种具有抗氧化和免疫调节特性的功能性食品,与有益的肠道微生物群组成相关。
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Antioxidant and immunomodulatory properties of aqueous acetic acid macromolecular extract from Saccharina japonica holdfast and its effects on the caecal microbiota of mice fed a high-sucrose and low-fibre diet

A large quantity of Saccharina japonica holdfast (SjH) is discarded as an unused by-product of the cultivation process. In this study, we isolated a functional fucoidan extract (FE) from SjH as an aqueous acetic acid macromolecular (>3 kDa) fraction. The main compounds in FE were fucoidans and phenolic compounds (phlorotannins). FE demonstrated notable superoxide anion radical-scavenging capacity in vitro, promoted nitric oxide (NO) secretion in RAW264.7 cells in the absence of lipopolysaccharide (LPS) from E. coli O111, and exerted an inhibitory effect on NO secretion in the presence of LPS. Mice were fed a high-sucrose diet containing no fibre (NF), 0.5% FE (FL) or 1.0% FE (FH) for 14 days. Compared with that in the spleen tissue of NF group mice, pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumour necrosis factor-α were suppressed in FL- and FH-fed mice. Interferon-γ was suppressed by FH. The caecal microbiota was analysed using 16S rDNA (V4) amplicon sequencing. Allobaculum- and Bacteroides acidifaciens–like bacteria were abundant in FL- and FH-fed mice. In contrast, the abundances of Desulfovibrionaceae, which are inflammation- and gut dysbiosis–related microbes, and Prevotellaceae-like bacteria were reduced in FH-fed mice. These results suggest that SjH FE is a functional food with antioxidant and immunomodulatory properties that correlates with beneficial gut microbiota composition.

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