微管相关蛋白7 (MAP7)微管结合域的共振配位

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2023-04-26 DOI:10.1007/s12104-023-10124-8
Agnes Adler, Lenette F. Kjaer, J. Wouter Beugelink, Marc Baldus, Hugo van Ingen
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引用次数: 1

摘要

微管相关蛋白7 (MAP7)是一种通过c端激酶结合域与激酶-1相互作用,参与沿微管(MTs)运输货物的蛋白质。此外,据报道,该蛋白稳定MT,从而在轴突分支发育中发挥关键作用。MAP7的112个氨基酸长n端微管结合域(MTBD)是后一种功能的重要组成部分。在这里,我们报告了核磁共振主链和侧链分配,表明该MTBD在溶液中主要是α -螺旋二级折叠。MTBD含有一个中心长的α-螺旋片段,其中包括一个短的四残基“铰链”序列,螺旋度降低,柔韧性增加。我们的数据代表了通过核磁共振光谱在原子水平上分析MAP7与mt的复杂相互作用的第一步。
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Resonance assignments of the microtubule-binding domain of the microtubule-associated protein 7 (MAP7)

The microtubule-associated protein 7 (MAP7) is a protein involved in cargo transport along microtubules (MTs) by interacting with kinesin-1 through the C-terminal kinesin-binding domain. Moreover, the protein is reported to stabilize MT, thereby playing a key role in axonal branch development. An important element for this latter function is the 112 amino-acid long N-terminal microtubule-binding domain (MTBD) of MAP7. Here we report NMR backbone and side-chain assignments that suggest a primarily alpha-helical secondary fold of this MTBD in solution. The MTBD contains a central long α-helical segment that includes a short four-residue ‘hinge’ sequence with decreased helicity and increased flexibility. Our data represent a first step towards analysing the complex interaction of MAP7 with MTs at an atomic level via NMR spectroscopy.

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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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