制定实时肺炎球菌定量PCR标准

Q1 Biochemistry, Genetics and Molecular Biology Biomolecular Detection and Quantification Pub Date : 2014-12-01 DOI:10.1016/j.bdq.2014.11.003

Susan C. Morpeth , Jim F. Huggett , David R. Murdoch , J. Anthony G. Scott

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引用次数: 8

摘要

定量lytA PCR通常使用内部标准进行。当通过菌落形成单位(CFU)或基因组拷贝测量肺炎链球菌的标准悬浮液时，我们假设等效。CFU/基因组拷贝的中位数(IQR)比为0.19(0.1-1.2)。基因组拷贝比CFU变化更小，但两种方法之间的差异凸显了绝对量化的挑战。

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Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

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