{"title":"几种自由放养动物在隐孢子虫多宿主传播中的作用。","authors":"Mehran Mirzaghavami, Javid Sadraei, Majid Pirestani, Saeed Bahadory","doi":"10.18502/ijpa.v18i3.13754","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>We aimed to characterize <i>Cryptosporidium</i> spp. in rats, cats, pigeons, and crows.</p><p><strong>Methods: </strong>Fifty-five animal origin <i>Cryptosporidium</i> spp. genome were identified, genotyped and confirmed by nested PCR and of RFLP-PCR analysis as well as sequenced based on 18s rRNA and <i>gp60</i> genes in Tehran (2012-2019). Finally, the phylogenetic analysis was performed by MEGA software (version 7).</p><p><strong>Results: </strong>By the molecular method, <i>Cryptosporidium</i> spp. were detected in 24 (15.2%), 15 (15%), 2 (2%) and 13 (13%) cases of wild rats, cat, pigeon, and crow, respectively. Among the identified species by the RFLP pattern, most isolates were identified as <i>C. parvum</i> (24/157) 17.8% in rats, (15/100) 15% in cats, (13/100) 13%in crew and (2/100) 2% in pigeons; and the rest of the cases were <i>C. muris</i> and <i>C. felis</i>. The results of sequencing did not prove the existence of <i>C. parvum</i>, <i>C. felis</i>, <i>C. muris</i>, and rat genotype. Subtyping of <i>C. parvum</i> was indicated that the dominant subtype family belongs to the IId family and the subtype A20G1 was the most common subtype detected in all hosts while A19G1 was detected in one isolate of cat and pigeon.</p><p><strong>Conclusion: </strong>Free-ranging animals are infected by species/subtype of <i>Cryptosporidium</i>, which can infect humans. This shows by itself the hygienic importance of the free-ranging animals in urban ecosystems. In the transmission of human cryptosporidiosis, the multi-host <i>Cryptosporidium</i> species such as <i>C. parvum</i>, <i>C. felis</i>, and <i>C. muris</i> can be transferred potentially from these animals to humans.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"18 3","pages":"313-323"},"PeriodicalIF":0.9000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/c9/IJPA-18-313.PMC10597881.pdf","citationCount":"0","resultStr":"{\"title\":\"The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of <i>Cryptosporidium</i> Spp.\",\"authors\":\"Mehran Mirzaghavami, Javid Sadraei, Majid Pirestani, Saeed Bahadory\",\"doi\":\"10.18502/ijpa.v18i3.13754\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>We aimed to characterize <i>Cryptosporidium</i> spp. in rats, cats, pigeons, and crows.</p><p><strong>Methods: </strong>Fifty-five animal origin <i>Cryptosporidium</i> spp. genome were identified, genotyped and confirmed by nested PCR and of RFLP-PCR analysis as well as sequenced based on 18s rRNA and <i>gp60</i> genes in Tehran (2012-2019). Finally, the phylogenetic analysis was performed by MEGA software (version 7).</p><p><strong>Results: </strong>By the molecular method, <i>Cryptosporidium</i> spp. were detected in 24 (15.2%), 15 (15%), 2 (2%) and 13 (13%) cases of wild rats, cat, pigeon, and crow, respectively. Among the identified species by the RFLP pattern, most isolates were identified as <i>C. parvum</i> (24/157) 17.8% in rats, (15/100) 15% in cats, (13/100) 13%in crew and (2/100) 2% in pigeons; and the rest of the cases were <i>C. muris</i> and <i>C. felis</i>. The results of sequencing did not prove the existence of <i>C. parvum</i>, <i>C. felis</i>, <i>C. muris</i>, and rat genotype. Subtyping of <i>C. parvum</i> was indicated that the dominant subtype family belongs to the IId family and the subtype A20G1 was the most common subtype detected in all hosts while A19G1 was detected in one isolate of cat and pigeon.</p><p><strong>Conclusion: </strong>Free-ranging animals are infected by species/subtype of <i>Cryptosporidium</i>, which can infect humans. This shows by itself the hygienic importance of the free-ranging animals in urban ecosystems. In the transmission of human cryptosporidiosis, the multi-host <i>Cryptosporidium</i> species such as <i>C. parvum</i>, <i>C. felis</i>, and <i>C. muris</i> can be transferred potentially from these animals to humans.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"18 3\",\"pages\":\"313-323\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/c9/IJPA-18-313.PMC10597881.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v18i3.13754\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v18i3.13754","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of Cryptosporidium Spp.
Background: We aimed to characterize Cryptosporidium spp. in rats, cats, pigeons, and crows.
Methods: Fifty-five animal origin Cryptosporidium spp. genome were identified, genotyped and confirmed by nested PCR and of RFLP-PCR analysis as well as sequenced based on 18s rRNA and gp60 genes in Tehran (2012-2019). Finally, the phylogenetic analysis was performed by MEGA software (version 7).
Results: By the molecular method, Cryptosporidium spp. were detected in 24 (15.2%), 15 (15%), 2 (2%) and 13 (13%) cases of wild rats, cat, pigeon, and crow, respectively. Among the identified species by the RFLP pattern, most isolates were identified as C. parvum (24/157) 17.8% in rats, (15/100) 15% in cats, (13/100) 13%in crew and (2/100) 2% in pigeons; and the rest of the cases were C. muris and C. felis. The results of sequencing did not prove the existence of C. parvum, C. felis, C. muris, and rat genotype. Subtyping of C. parvum was indicated that the dominant subtype family belongs to the IId family and the subtype A20G1 was the most common subtype detected in all hosts while A19G1 was detected in one isolate of cat and pigeon.
Conclusion: Free-ranging animals are infected by species/subtype of Cryptosporidium, which can infect humans. This shows by itself the hygienic importance of the free-ranging animals in urban ecosystems. In the transmission of human cryptosporidiosis, the multi-host Cryptosporidium species such as C. parvum, C. felis, and C. muris can be transferred potentially from these animals to humans.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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