成纤维细胞生长因子8剪接异构体b的核磁共振分配

IF 0.8 4区生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2023-04-29 DOI:10.1007/s12104-023-10132-8

Bruno Hargittay, Konstantin S. Mineev, Christian Richter, Sridhar Sreeramulu, Hendrik R.A. Jonker, Krishna Saxena, Harald Schwalbe

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引用次数: 0

摘要

人成纤维细胞生长因子8 (FGF8b)剪接异构体b是脑胚胎发育的重要调控因子。在这里，我们报告了FGF8b的主链和脂肪侧链的几乎完整的核磁共振化学位移分配。得到的化学位移与先前报道的x射线数据很好地一致，不包括n端gN螺旋，它显然只与受体形成复合物。报道的数据为研究FGF8b与其受体和潜在药物或抑制剂的相互作用提供了一个核磁共振起点。

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NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b

The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.