Li Fu-heng , Xu Qing-hua , A B ПOЛяKOB , Qu Di , Zhu Hui-jie , Li Nan-ding , Liu Zeng-bing
{"title":"金银花最佳分化培养基的筛选","authors":"Li Fu-heng , Xu Qing-hua , A B ПOЛяKOB , Qu Di , Zhu Hui-jie , Li Nan-ding , Liu Zeng-bing","doi":"10.1016/S1006-8104(15)30016-7","DOIUrl":null,"url":null,"abstract":"<div><p>An excellent <em>Lonicera edulis</em> strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A four-factor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of <em>Lonicera edulis</em> micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L<sup>−1</sup> 6-BA, 0.3 mg · L<sup>−1</sup> IBA and 1.5 mg · L<sup>−1</sup> GA<sub>3</sub>.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(15)30016-7","citationCount":"1","resultStr":"{\"title\":\"Screening of Optimal Differentiation Medium to Lonicera edulis\",\"authors\":\"Li Fu-heng , Xu Qing-hua , A B ПOЛяKOB , Qu Di , Zhu Hui-jie , Li Nan-ding , Liu Zeng-bing\",\"doi\":\"10.1016/S1006-8104(15)30016-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An excellent <em>Lonicera edulis</em> strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A four-factor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of <em>Lonicera edulis</em> micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L<sup>−1</sup> 6-BA, 0.3 mg · L<sup>−1</sup> IBA and 1.5 mg · L<sup>−1</sup> GA<sub>3</sub>.</p></div>\",\"PeriodicalId\":58038,\"journal\":{\"name\":\"Journal of Northeast Agricultural UniversityEnglish Edition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1006-8104(15)30016-7\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Northeast Agricultural UniversityEnglish Edition\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1006810415300167\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Northeast Agricultural UniversityEnglish Edition","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1006810415300167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Screening of Optimal Differentiation Medium to Lonicera edulis
An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A four-factor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L−1 6-BA, 0.3 mg · L−1 IBA and 1.5 mg · L−1 GA3.
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