东亚哺乳动物分子DNA系统分析:韩国普通水獭(哺乳动物,食肉目)细胞色素b基因序列变异及线粒体DNA控制区

H. Koh, Minyoung Yoo, Bae Geun Lee, Jeong Gyu Park
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引用次数: 7

摘要

对韩国水獭(Lutra Lutra Lutra L.)的细胞色素b基因序列和线粒体DNA控制区进行了分析,以期为该亚种的保护提供遗传信息。细胞色素b基因和控制区分别显示2个单倍型和1个单倍型。将GenBank中欧洲普通水獭(l.l.l utra)的可用序列与韩国普通水獭的序列进行比较,以确定它们之间的序列差异程度。在细胞色素b基因序列中,韩国的2个单倍型和欧洲的2个单倍型在1045个位点中有12个存在差异。两种欧洲单倍型之间的Tamura - Nei核苷酸距离为0.10%,两种韩国单倍型之间的Tamura - Nei核苷酸距离为0.10%,而韩国单倍型与欧洲单倍型之间的Tamura - Nei核苷酸距离为0.96% ~ 1.16%。在对照区,300个位点中有7个位点出现了1个韩国单倍型和7个欧洲单倍型的差异;7个欧洲单倍型的Tamura - Nei距离为0.34% ~ 1.01%,而韩国单倍型与欧洲单倍型的Tamura - Nei距离为1.01% ~ 1.69%。虽然需要对包括黑龙江地区和中国东北在内的东亚地区的标本进行进一步的分子和形态学研究,但韩国普通水獭可能与远东普通水獭l.i. amurensis Dybowski更接近或相同。
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Molecular DNA systematic analyses of East Asian mammals: Sequence Variation of cytochrome b Gene and Control Region of Mitochondrial DNA of Common Otter, Lutra lutra lutra L. (Mammalia, Carnivora) from Korea
Sequences of cytochrome b gene and control region of mitochondrial DNA from Korean common otters (Lutra lutra lutra L.) were examined to provide the genetic information for the conservation of this subspecies. Two haplotypes and one haplotype were revealed in cytochrome b gene and control region, respectively. The available sequences of European common otter (L. l. lutra) from GenBank were compared together with those of Korean common otter in order to determine the degree of sequence variation between them. In cytochrome b gene sequences, two haplotypes from Korea and two haplotypes of Europe showed differences in 12 of 1,045 sites. The Tamura‐Nei nucleotide distances between two European haplotypes was 0.10% and those between two Korean haplotypes was also 0.10%, but those between Korean haplotypes and European ones ranged from 0.96% to 1.16%. In the control region, one Korean haplotype and seven European ones showed differences in seven of 300 sites; the Tamura‐Nei distances among seven European haplotypes were 0.34% to 1.01%, but those between Korean haplotype and European ones ranged from 1.01% to 1.69%. Although further molecular and morphological studies with specimens from eastern Asia including Amur region and northeast China are needed, it is possible that the Korean common otter might be closer or identical to the far‐eastern Asian common otter, L. I. amurensis Dybowski.
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