Korean journal of biological sciences最新文献

英文中文

Six SIGMA evaluation of 17 biochemistry parameters using bias calculated from internal quality control and external quality assurance data. 利用内部质量控制和外部质量保证数据计算出的偏差，对 17 个生化参数进行六标法评估。

IF 2.5

Korean journal of biological sciences

Pub Date : 2024-01-25 DOI: 10.5937/jomb0-43052

Tülay Çevlik, Goncagül Haklar

Background: Six Sigma is a popular quality management system that enables continuous monitoring and improvement of analytical performance in the clinical laboratory. We aimed to calculate sigma metrics and quality goal index (QGI) for 17 biochemical analytes and compare the use of bias from internal quality control (IQC) and external quality assurance (EQA) data in the calculation of sigma metrics.

Methods: This retrospective study was conducted in Marmara University Pendik E&R Hospital Biochemistry Laboratory. Sigma metrics calculation was performed as (TEa-bias)/CV). CV was calculated from IQC data from June 2018 - February 2019. EQA bias was calculated as the mean of % deviation from the peer group means in the last seven surveys, and IQC bias was calculated as (laboratory control result mean-manufacturer control mean)/ manufacturer control mean) x100. In parameters where sigma metrics were <5; QGI=bias/1.5 CV) score of <0.8 indicated imprecision, >1.2 pointed inaccuracy, and 0.8-1.2 showed both imprecision and inaccuracy.

Results: Creatine kinase (both levels), iron and magnesium (pathologic levels) showed an ideal performance with ≥6 sigma level for both bias determinations. Eight of the 17 parameters had different sigma levels when we compared sigma values calculated from EQA and IQC derived bias% while the rest were grouped at the same levels.

Conclusions: Sigma metrics is a good quality tool to assess a laboratory's analytical performance and facilitate the comparison of the assay performances in the same manner across multiple systems. However, we might need to design a tight internal quality control protocol for analytes showing poor assay performance.

背景：六西格玛是一种流行的质量管理系统，可持续监控和改进临床实验室的分析性能。我们旨在计算 17 种生化分析物的西格玛指标和质量目标指数（QGI），并比较内部质量控制（IQC）和外部质量保证（EQA）数据在计算西格玛指标时的偏差使用情况：这项回顾性研究在马尔马拉大学 Pendik E&R 医院生化实验室进行。西格玛指标的计算方法为 (TEa-bias)/CV) 。CV 根据 2018 年 6 月至 2019 年 2 月的 IQC 数据计算。EQA 偏差按最近七次调查中与同行组平均值偏差的百分比平均值计算，IQC 偏差按（实验室对照结果平均值-制造商对照平均值）/制造商对照平均值）x100 计算。在西格玛指标为 1.2 的参数中，不准确度为 0.8-1.2，不精确度和不准确度均为 0.8-1.2：结果：肌酸激酶（两个水平）、铁和镁（病理水平）的性能理想，两个偏差测定的西格玛水平均≥6。当我们比较根据 EQA 和 IQC 得出的偏倚率计算的西格玛值时，17 个参数中有 8 个参数的西格玛值不同，而其余参数的西格玛值相同：西格玛指标是评估实验室分析性能的良好质量工具，有助于以相同的方式比较多个系统的检测性能。不过，我们可能需要针对检测性能不佳的分析物设计一套严格的内部质量控制方案。

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引用次数: 0

Identification of Ku70/Ku80 as ADD1/SREBP1c interacting proteins Ku70/Ku80为ADD1/SREBP1c互作蛋白的鉴定

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647733

Y. S. Lee, Hae‐Young Koh, S. Park, J. B. Kim

In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell‐type‐ and gene‐specific DNA‐binding proteins in a chromatin‐selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin‐dependent gene expression through binding to SRE and E‐box motif with dual DNA binding specificity. Although its transcriptional and post‐translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SREBP1c, we employed the GST pull‐down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREBP1c protein. GST pull‐down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co‐transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.

在脊椎动物中，多亚基辅助因子通过与细胞类型和基因特异性DNA结合蛋白以染色质选择性方式相互作用来调节基因表达。ADD1/SREBP1c通过与SRE和E - box基序结合，具有双DNA结合特异性，调节脂肪酸代谢和胰岛素依赖性基因表达。尽管其转录和翻译后调控已被广泛研究，但其通过蛋白质相互作用的调控尚不清楚。为了鉴定与ADD1/SREBP1c核形式相关的细胞蛋白，我们使用了Hela细胞核提取物的GST下拉系统。在这项研究中，我们证明了Ku蛋白与ADD1/SREBP1c蛋白特异性相互作用。GST pull - down结合肽段测序分析显示，Ku80在体外与ADD1/SREBP1c结合。此外，western blot分析显示Ku80的异二聚化伴侣Ku70也与ADD1/SREBP1c相关。此外，与ADD1/SREBP1c共转染Ku70/Ku80可增强ADD1/SREBP1c的转录活性。综上所述，这些结果表明Ku蛋白可能通过与ADD1/SREBP1c相互作用参与脂肪生成和/或脂肪生成基因的表达。

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引用次数: 1

Three new species of collocheres (copepoda, siphonostomatoida, asterocheridae) associated with crinoids and ophiuroids from Korea 标题韩国菊科和蛇科菊科三新种(桡足目、单足目、星足科)

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647760

Sook Shin, Il-Hoi Kim

Three new species of Collocheres are described as associates of crinoid and ophiuroid echinoderms from Cheju Island, Korea: Collocheres brevipes n. sp. from the crinoids Comanthus solaster A. H. Clark and Decametra tigrina (A. H. Clark); C. solidus n. sp. from two species of the crinoids Comanthus solaster and Comanthus japonicus (Müller) and one species of ophiuroid‐Ophiomastax mixta (Lütken); and C. tamladus n. sp. from the crinoid Catoptometra rubroflava (A. H. Clack). The three species are distinguished from one another and other congeners by the body size, dimension of caudal ramus and free segment of leg 5, and shape and ornamentation of urosome.

报道了韩国州岛海百合和蛇属棘皮动物的3个新种:海百合科Comanthus solaster A. H. Clark和Decametra tigrina (A. H. Clark)中的Collocheres brevipes n. sp;产自海百合科(Comanthus solaster)和日本科(Comanthus japonicus)两种，产自蛇麻-蛇麻混合科(l tken)一种;和C. tamladus n. sp来自海百合Catoptometra rubroflava (A. H. Clack)。这三种的体长、尾支和第5条腿的自由节的大小、尾小体的形状和纹饰是它们与其他同类的区别。

引用次数: 1

Ventral striatal connections of unimodal and multimodal cortex of the superior temporal sulcus in macaque monkeys(macacca nemestrina) 猕猴颞上沟皮层单峰和多峰的腹侧纹状体连接

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647767

Y. Jung, Sungwon J. Hong

Extrinsic connections between the cortex of the superior temporal sulcus (STS) and the ventral striatum in pigtail macaque monkeys (Macacca nemestrina) were studied by injection of retrograde tracers into the ventromedial caudate nucleus, the ventral and central shells of the nucleus accumbens (NA), the dorsal core of the NA, and the ventrolateral putamen. In the present study, we demonstrate that the projections from the unimodal (area TAa, IPa, TEa, and TEm) and the multimodal (area TPO and PGa) sensory association areas in the STS mainly terminate in the ventromedial caudate nucleus as well as in the ventral and central shells of the NA. However, there are only few projections to the dorsal core of the NA and the ventrolateral putamen from the sensory association cortex in the STS. Based on these differential neural connections between the subterritories of the ventral striatum and the sensory association areas, the ventromedial caudate nucleus and the shells of NA appear to be major integration sites for sensory input from the STS and functionally different from the dorsal core of NA and the ventrolateral putamen.

通过向尾状核腹内侧、伏隔核腹侧和中央壳、伏隔核背核和腹外侧壳核注射逆行示踪剂，研究了长尾猕猴颞上沟皮层与腹侧纹状体的外在连接。在本研究中，我们证明了STS中单峰区(TAa, IPa, TEa和TEm区)和多峰区(TPO和PGa区)感觉关联区的投射主要终止于腹内侧尾状核以及NA的腹侧和中央壳。然而，在STS中，感觉联合皮层对NA背核和腹外侧壳核的投射很少。基于腹侧纹状体亚区和感觉关联区之间的这些不同神经连接，腹内侧尾状核和NA的外壳似乎是STS感觉输入的主要整合位点，在功能上与NA的背核和腹外壳核不同。

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引用次数: 1

Tra2α and hnRNP K might be functional partners of Rbm for regulation of RNA processes during spermatogenesis Tra2α和hnRNP K可能是Rbm在精子发生过程中调控RNA过程的功能伙伴

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647735

Jungmin Lee, Euisu Kim, S. Jang, K. Rhee

Rbm is a male infertility gene located in the AZFb region of the Y chromosome. Expression pattern of Rbm indicates that Rbm is critical for early phase of male germ cell development. It shares strong structural homology with hnRNP G, suggesting a function as an RNA processing factor. In order to gain a clue on the molecular mechanisms of Rbm on male germ cell development, we examined interactions of Rbm with selected proteins in yeast. The results revealed specific interactions between Rbm, hnRNP K and Tra2α. These results suggest that hnRNP K and Tra2α may be functional partners of Rbm in male germ cells. We propose a model in which hnRNP K may play a role as a platform for Rbm and Tra2α.

Rbm是一种位于Y染色体AZFb区域的男性不育基因。Rbm的表达模式表明Rbm在男性生殖细胞发育的早期阶段起关键作用。它与hnRNP G具有强烈的结构同源性，表明其具有RNA加工因子的功能。为了进一步了解Rbm对男性生殖细胞发育的分子机制，我们研究了Rbm与酵母中选定蛋白的相互作用。结果揭示了Rbm、hnRNP K和Tra2α之间的特异性相互作用。这些结果提示hnRNP K和Tra2α可能是雄性生殖细胞中Rbm的功能伴侣。我们提出了一个hnRNP K可能作为Rbm和Tra2α平台的模型。

引用次数: 1

Copepodid stages of Ergasilus hypomesi Yamaguti (Copepoda, Poecilostomatoida, Ergasilidae) from a brackish lake in Korea 韩国某咸淡湖泊中拟沙螽桡足期(桡足目，拟沙螽科，拟沙螽科)

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647727

Il-Hoi Kim

All copepodid stages and free‐swimming adults of Ergasilus hypomesi Yamaguti collected from plankton samples from a brackish lake in Korea are described. The antennule remains a four‐segmented appendage until copepodid V. The antenna of copepodid I carries a vestigial exopod. Sexual dimorphism first appears at copepodid IV whose males bear primodial maxillipeds. Leg 5 also is sexually dimorphic from copepodid IV onwards, with its free segment bearing three setae in the female and two setae in the male. Morphological features of some appendages appearing during copepodid development are discussed. It is noticed that the early copepodids of the Ergasilidae show the characteristic setation on the biramous legs and proximal segments of the antennule.

本文描述了从韩国咸淡湖浮游生物样本中收集的所有桡足动物阶段和自由游动的山口水蚤成虫。桡足类I的触角带有一个退化的外足。两性二态性首先出现在第四桡足动物，其雄性具有原始的上足动物。从第四桡足动物开始，第5条腿也是两性二态的，其自由节雌性有3根刚毛，雄性有2根刚毛。讨论了桡足动物发育过程中出现的一些附属物的形态特征。值得注意的是，早期的叶甲科桡足类在双足和触须近端显示出特有的位置。

引用次数: 5

Taxonomic notes on some Korean species of the genus ampelisco (amphipoda, ampeliscidae) 韩国蛇足属若干种的分类学注释(片足目，蛇足科)

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647759

Young Hyo Kim, Y. Eun, Kyung-Sook Lee

While examining specimens collected in the shallow waters of Korea from 1995 to 2004, we found that Ampelisca bocki Dahl and A. naikaiensis Nagata were newly added to Korean fauna. We also provide description for A brevicornis (Costa) based on materials collected from Korea. As a result, six species of Ampelisca are reported in Korea.

对1995 ~ 2004年在韩国浅海采集的标本进行分析，发现韩国的区系中新增了Ampelisca bocki Dahl和A. naikaiensis Nagata。我们还根据韩国收集的资料提供了A brevicornis (Costa)的描述。因此，在国内发现了6种Ampelisca。

引用次数: 1

Effect of host egg color dimorphism on interactions between the vinous‐throated parrotbill (Paradoxornis webbianus) and common cuckoo (Cuculus canorus) 寄主卵颜色二态性对毒喉鹦鹉与普通杜鹃相互作用的影响

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647737

Jin‐Won Lee, Jeong-Chil Yoo

To investigate the effect of host egg color dimorphism on the vinous‐throated parrotbill (Paradoxornis webbianus) – common cuckoo (Cuculus canorus) interactions, we monitored breeding nests of vinous‐throated parrotbills, and conducted model egg experiments, using two colors: white and blue. Of the 190 nests examined in this study, cuckoo parasitism occurred at 10 nests (8 blue and 2 white egg clutches, respectively), and only blue cuckoo eggs were found. This frequency was similar to the egg‐color ratio of all host nests found (151 blue and 39 white egg clutches). Vinous‐throated parrotbills showed high rejection rate towards both cuckoo eggs and model ones. There was a significant difference in rejection rates towards mimetic (blue) and non‐mimetic (white) eggs in blue egg clutches. Mimetic eggs put in nests took significantly longer to be rejected than non‐mimetic ones. The most common rejection method used by the hosts was egg ejection (puncture‐ejection). The costs of ejecting non‐mimetic eggs tended to be lower than those of ejecting mimetic eggs. These results indicate that egg‐color dimorphism in this species favors the individuals having white egg clutches in terms of higher rejection rate and lower ejection costs of the parasitic eggs. This study also suggests that egg‐color dimorphism of the vinous‐throated parrotbill decreases the effect of cuckoo parasitism on host populations.

为了研究寄主蛋颜色二态性对毒喉鹦鹉(Paradoxornis webbianus) -普通杜鹃(Cuculus canorus)相互作用的影响，我们对毒喉鹦鹉的繁殖巢进行了监测，并使用白色和蓝色两种颜色进行了模型蛋实验。在本研究调查的190个巢中，有10个巢(分别为8个蓝色和2个白色的卵窝)发生了杜鹃寄生，只有蓝色的杜鹃卵被发现。这一频率与发现的所有寄主巢的蛋色比相似(151个蓝色卵窝和39个白色卵窝)。酒喉鹦鹉对杜鹃蛋和模型鸟的排异率都很高。在蓝卵窝中，对仿卵(蓝色)和非仿卵(白色)的排异率有显著差异。放入巢中的模拟蛋被排斥的时间明显长于非模拟蛋。宿主最常用的排斥方法是射卵(穿刺-射卵)。射出非模拟卵的成本往往低于射出模拟卵的成本。这些结果表明，该物种的卵色二态性在较高的排斥率和较低的排出成本方面有利于拥有白色卵的个体。该研究还表明，毒喉鹦鹉的卵色二态性降低了杜鹃寄生对寄主种群的影响。

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引用次数: 19

Relationships between methane production and sulfate reduction in reclaimed rice field soils 复垦稻田土壤甲烷产量与硫酸盐还原的关系

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647761

Ju-hwan Lee, Kang-Hyun Cho

The change in relationships between methane production and sulfate reduction was investigated in reclaimed rice field soils at different time points after reclamation of tidal flat in Korea. Sulfate concentrations of soils in the ca. 60‐year‐old and 26‐year‐old reclaimed rice fields were much lower than that in a natural tidal flat. During 60 d of anaerobic incubation, total methane production and sulfate consumption of the soil slurries were 7.0 μmol CH4/g and 8.2 μnol SO4 2‐/g in the 60‐year‐old rice field, 5.6 μmol CH4/g and 12.7 μmol SO4 2‐/g in the 26‐year‐old rice field, and ca. 0 μmol CH4/g and 22.4 μmol SO4 2‐/g in a natural tidal flat. Relative percent electron flow through sulfate reduction in the 60‐year‐old rice field was much lower (50.8%) compared with the 26‐year‐old rice field (69.3%) and the tidal flat (99.9%). The addition of an inhibitor of methanogenesis (2‐bromoethanesulfonate) had no effect on sulfate reduction in the soil slurries of the reclaimed rice fields. However, instant stimulation of methane production was achieved with addition of an inhibitor of sulfate reduction (molybdate) in the soil slurries from the 26‐year‐old reclaimed rice field. The specific inhibitor experiments suggest that the relationship of methanogenesis and sulfate reduction might become mutually exclusive or syntrophic depending on sulfate content in the soil after reclamation. Sulfate, thus sulfate reduction activity of sulfate‐reducing bacteria, would be an important environmental factor that inhibits methane production and determines the major pathway of electron and carbon flow in anaerobic carbon mineralization of reclaimed rice field soils.

研究了韩国潮滩复垦后不同时间点稻田土壤甲烷产量与硫酸盐还原关系的变化。60年和26年稻田土壤中硫酸盐含量明显低于自然潮滩。在60 d的厌氧培养过程中，60年稻田土壤污泥的总甲烷产量和硫酸盐消耗量分别为7.0 μmol CH4/g和8.2 μmol SO4 2‐/g, 26年稻田土壤污泥的总甲烷产量和硫酸盐消耗量分别为5.6 μmol CH4/g和12.7 μmol SO4 2‐/g，天然潮滩土壤污泥的总甲烷产量和硫酸盐消耗量分别为0 μmol CH4/g和22.4 μmol SO4‐/g。60年稻田通过硫酸盐还原的相对电子流比例(50.8%)远低于26年稻田(69.3%)和潮滩(99.9%)。添加甲烷生成抑制剂(2‐溴乙烷磺酸)对复垦稻田土壤浆液中硫酸盐的还原没有影响。然而，通过在26年的复垦稻田的土壤浆液中添加硫酸盐还原抑制剂(钼酸盐)，可以立即提高甲烷产量。特定抑制剂实验表明，开垦后土壤中硫酸盐含量的不同，甲烷生成与硫酸盐还原的关系可能是互斥的或共生的。硫酸盐以及硫酸盐还原菌的硫酸盐还原活性是抑制甲烷生成的重要环境因子，并决定了复垦稻田土壤厌氧碳矿化过程中电子和碳流的主要途径。

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引用次数: 0

Reproductive cycle and spawning rhythm of the ascidian, Halocynthia hilgendorfi ritteri 海鞘的生殖周期和产卵节律

Korean journal of biological sciences

Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647731

Young-jin Choi, Chi‐Hoon Lee, S. Rho, Young-Don Lee

Reproductive cycle and spawning rhythm with lunar cycle of the ascidian, Halocynthia hilgendorfi ritteri were investigated by histological examination. The specimens were sampled in the coastal waters of Yongdam, northwest of Jeju Island, Korea, from November 2001 to January 2003. H. hilgendorfi ritteri is a synchronous hermaphrodite; the gonads are located in the mantle. The reproductive cycle can be grouped into the following successive stages in the ovary: growth (February to June), vitellogenesis (April to September), mature (July to December), spent (November to February), and recovery (December to April). Likewise, in the testis, the stages observed were: growth (October), mature (October to December), spent (November to February), and resting (January to September). Major spawning probably occurs between November and January, when water temperatures decrease. The histological observations of the gonads suggested that this species is a multiple spawner during the spawning period. Spawning occurred between the new moon and full moon, and again between the full moon and new moon, suggesting that the spawning rhythm is influenced by the lunar cycle.

用组织学方法研究了海鞘的生殖周期和产卵节律与月相的关系。这些标本于2001年11月至2003年1月在韩国济州岛西北部龙潭沿海水域取样。H. hilgendorfi ritteri是同步雌雄同体;性腺位于地幔中。卵巢的生殖周期可分为以下几个连续的阶段:生长(2月至6月)、卵黄形成(4月至9月)、成熟(7月至12月)、花(11月至2月)和恢复(12月至4月)。同样，在睾丸中，观察到的阶段是:生长(10月)，成熟(10月至12月)，花(11月至2月)和休息(1月至9月)。主要的产卵可能发生在11月到1月之间，此时水温下降。性腺的组织学观察表明，该物种在产卵期是一个多产卵者。产卵发生在新月和满月之间，满月和新月之间，这表明产卵节奏受到月亮周期的影响。

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引用次数: 7

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